Somatostatin Interneurons Delineate the Inner Part of the External Plexiform Layer in the Mouse Main Olfactory Bulb
Somatostatin Interneurons Delineate the Inner Part of the External Plexiform Layer in the Mouse Main Olfactory Bulb
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DOI：
10.1002/cne.22317
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发表时间：
2010-06-01
影响因子：
2.5
通讯作者：
Viollet, Cecile
中科院分区：
文献类型：
--
作者：
Lepousez, Gabriel;Csaba, Zsolt;Viollet, Cecile
神经肽在神经网络的信息处理中起着重要的调节作用。生长抑素是大脑中浓度最高的神经肽之一，存在于包括嗅觉通路在内的许多感觉系统中。然而，其在小鼠主嗅球（MOB）中的细胞分布尚不清楚。在这里，我们发现大约95%的小鼠球生长抑素免疫反应（SRIF-ir）细胞描述了一个均匀的中间神经元群体。这些局限于外网状层（iEPL）的内层，树突场严格限制在该区域。iEPL SRIF-ir神经元具有Van Gehuchten短轴突细胞的部分形态特征，均表达谷氨酸脱羧酶、calretinin和血管活性肠肽。一半的SRIF-ir神经元是小蛋白-ir，与其他前脑区域（如皮层或海马）的SRIF-ir中间神经元相比，显示出非典型的神经化学特征。生长抑素也存在于纤维和颗粒细胞层（GCL）的一些稀疏的假定的深短轴突细胞中，这在其他哺乳动物物种中也有报道。生长抑素中间神经元在MOB iEPL中的空间分布清楚地勾勒出二尖瓣细胞侧树突通过树突互交突触与GCL抑制性中间神经元相互作用的区域。对称和不对称的突触接触发生在SRIF-ir树突和二尖瓣细胞树突之间。这种生长抑素中间神经元的限制性定位和球突触网络的连通性强烈表明，这种肽在嗅觉加工的调节中起着功能性作用。[j] .中国生物医学工程学报，2016,18(5):776 - 794。(C) 2010 Wiley-Liss, Inc。
Neuropeptides play a major role in the modulation of information processing in neural networks. Somatostatin, one of the most concentrated neuropeptides in the brain, is found in many sensory systems including the olfactory pathway. However, its cellular distribution in the mouse main olfactory bulb (MOB) is yet to be characterized. Here we show that approximate to 95% of mouse bulbar somatostatin-immunoreactive (SRIF-ir) cells describe a homogeneous population of interneurons. These are restricted to the inner lamina of the external plexiform layer (iEPL) with dendritic field strictly confined to the region. iEPL SRIF-ir neurons share some morphological features of Van Gehuchten short-axon cells, and always express glutamic acid decarboxylase, calretinin, and vasoactive intestinal peptide. One-half of SRIF-ir neurons are parvalbumin-ir, revealing an atypical neuro-chemical profile when compared to SRIF-ir interneurons of other forebrain regions such as cortex or hippocampus. Somatostatin is also present in fibers and in a few sparse presumptive deep short-axon cells in the granule cell layer (GCL), which were previously reported in other mammalian species. The spatial distribution of somatostatin interneurons in the MOB iEPL clearly outlines the region where lateral dendrites of mitral cells interact with GCL inhibitory interneurons through dendrodendritic reciprocal synapses. Symmetrical and asymmetrical synaptic contacts occur between SRIF-ir dendrites and mitral cell dendrites. Such restricted localization of somatostatin interneurons and connectivity in the bulbar synaptic network strongly suggest that the peptide plays a functional role in the modulation of olfactory processing. J. Comp. Neurol. 518:1976-1994, 2010. (C) 2010 Wiley-Liss, Inc.