Pluripotent Stem Cells for Retinal Tissue Engineering: Current Status and Future Prospects.
Pluripotent Stem Cells for Retinal Tissue Engineering: Current Status and Future Prospects.
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DOI：
10.1007/s12015-018-9802-4
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发表时间：
2018-08
影响因子：
4.8
通讯作者：
Nasonkin IO
中科院分区：
文献类型：
--
作者：
Singh R;Cuzzani O;Binette F;Sternberg H;West MD;Nasonkin IO
视网膜是一个非常精细和分层的神经组织，它在很大程度上依赖于细胞、结构、连通性和脉管系统的保存来维持视力。迫切需要找到技术和生物解决方案，以解决与视网膜细胞功能替代相关的重大挑战。尚未解决的主要挑战包括产生足够数量的特定细胞类型，实现移植细胞（特别是光感受器）的功能整合，以及在不引发免疫反应、炎症和/或重塑的情况下手术递送视网膜细胞或组织。再生医学的进步使三维组织（类器官）的产生成为可能，部分地再现了几种组织的解剖结构、生物复杂性和生理学，这是干细胞替代疗法的重要目标。在培养皿中提取视网膜组织为失明的细胞替代疗法创造了新的机会，并解决了保存视网膜结构以恢复视力的需要。在过去的十年里，旨在保护和改善视力的视网膜细胞疗法取得了许多进展。视网膜类器官技术为视网膜细胞功能替代相关的技术和生物学挑战提供了许多解决方案，以实现长期视力恢复。我们总结了视网膜细胞治疗的进展，重点是人类多能干细胞来源的视网膜组织，并批判性地评估了视网膜类器官方法在解决视网膜变性和眼外伤引起的视网膜修复和视力恢复这一主要未满足的临床需求方面的潜力。我们还分析了视网膜类器官技术用于临床应用的商业化障碍。
The retina is a very fine and layered neural tissue, which vitally depends on the preservation of cells, structure, connectivity and vasculature to maintain vision. There is an urgent need to find technical and biological solutions to major challenges associated with functional replacement of retinal cells. The major unmet challenges include generating sufficient numbers of specific cell types, achieving functional integration of transplanted cells, especially photoreceptors, and surgical delivery of retinal cells or tissue without triggering immune responses, inflammation and/or remodeling. The advances of regenerative medicine enabled generation of three-dimensional tissues (organoids), partially recreating the anatomical structure, biological complexity and physiology of several tissues, which are important targets for stem cell replacement therapies. Derivation of retinal tissue in a dish creates new opportunities for cell replacement therapies of blindness and addresses the need to preserve retinal architecture to restore vision. Retinal cell therapies aimed at preserving and improving vision have achieved many improvements in the past ten years. Retinal organoid technologies provide a number of solutions to technical and biological challenges associated with functional replacement of retinal cells to achieve long-term vision restoration. Our review summarizes the progress in cell therapies of retina, with focus on human pluripotent stem cell-derived retinal tissue, and critically evaluates the potential of retinal organoid approaches to solve a major unmet clinical need—retinal repair and vision restoration in conditions caused by retinal degeneration and traumatic ocular injuries. We also analyze obstacles in commercialization of retinal organoid technology for clinical application.
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影响因子：
5.9
作者：
Assawachananont, Juthaporn;Mandai, Michiko;Okamoto, Satoshi;Yamada, Chikako;Eiraku, Mototsugu;Yonemura, Shigenobu;Sasai, Yoshiki;Takahashi, Masayo
通讯作者：
Takahashi, Masayo
影响因子：
4.6
作者：
Barnea-Cramer AO;Wang W;Lu SJ;Singh MS;Luo C;Huo H;McClements ME;Barnard AR;MacLaren RE;Lanza R
通讯作者：
Lanza R
DOI：
10.3791/53927
发表时间：
2016-09-13
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Al-Nawaiseh S;Thieltges F;Liu Z;Strack C;Brinken R;Braun N;Wolschendorf M;Maminishkis A;Eter N;Stanzel BV
通讯作者：
Stanzel BV
DOI：
10.1073/pnas.222546999
发表时间：
2002-11-12
影响因子：
11.1
作者：
Braz, JM;Rico, B;Basbaum, AI
通讯作者：
Basbaum, AI
影响因子：
16.6
作者：
Benedicto I;Lehmann GL;Ginsberg M;Nolan DJ;Bareja R;Elemento O;Salfati Z;Alam NM;Prusky GT;Llanos P;Rabbany SY;Maminishkis A;Miller SS;Rafii S;Rodriguez-Boulan E
通讯作者：
Rodriguez-Boulan E