Dissimilarities between cholinergic and dopaminergic turning elicited by nucleus accumbens stimulation in freely moving rats.
Dissimilarities between cholinergic and dopaminergic turning elicited by nucleus accumbens stimulation in freely moving rats.
复制标题
自由活动大鼠伏隔核刺激引起的胆碱能和多巴胺能转变之间的差异。
DOI：
10.1016/0014-2999(94)00741-o
复制
发表时间：
1995
影响因子：
5
通讯作者：
A. Cools
中科院分区：
文献类型：
--
作者：
T. Saigusa;N. Koshikawa;M. Kitamura;K. Mizutani;M. Kobayashi;A. Cools
对侧转动是通过将卡巴胆碱（0.5、2.5、5μg）单侧注射到伏隔核中产生的，但不注射到背侧或腹侧纹状体中。这种行为可以通过伏隔核中的毒蕈碱 M1 乙酰胆碱受体阻断来抑制，而通过阻断毒蕈碱 M2 和烟碱乙酰胆碱受体则效果较差。单侧注射多巴胺 D1 受体激动剂 1-苯基-2,3,4,5-四氢-1H-3-苯并氮杂-7,8-二醇（SKF 38393，5 μg）和多巴胺 D2 受体激动剂喹吡罗（10 μg）的混合物也产生对侧转向。然而，迈步模式与卡巴胆碱诱导的迈步模式完全不同。伏隔核中多巴胺 D1 或 D2 受体阻断（分别为 8-氯-2,3,4,5-四氢-3-甲基-5-苯基-1H-3-苯并氮杂-7-醇（SCH 23390）和 l-舒必利）可减少卡巴胆碱诱导的转动次数。然而，减少是由于转弯模式的变化。伏隔核中毒蕈碱乙酰胆碱受体的阻断不会改变伏隔核单侧注射多巴胺受体激动剂引起的对侧转向。结果表明，尽管多巴胺受体的阻断引起了与卡巴胆碱引起的转变竞争的行为缺陷，但所涉及的胆碱能底物和多巴胺能底物之间不存在功能性相互作用。将卡巴胆碱注射到伏隔核中引起的对侧转向需要同侧腹外侧纹状体中多巴胺 D1 和 D2 受体水平的完整多巴胺活性，但不需要对侧腹外侧纹状体。
Contralateral turning was produced by unilateral injection of carbachol (0.5, 2.5, 5 μg) into the nucleus accumbens, but not into the dorsal or ventral striatum. This behaviour was inhibited by muscarinic M1acetylcholine receptor blockade in the nucleus accumbens, and less effectively by blockade of muscarinic M2and nicotinic acetylcholine receptors. Unilateral injection of a mixture of the dopamine D1receptor agonist 1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine-7,8-diol (SKF 38393, 5 μg) and the dopamine D2receptor agonist quinpirole (10 μg) also produced contralateral turning. The stepping pattern, however, completely differed from that induced by carbachol. The number of carbachol-induced turnings was reduced by dopamine D1or D2receptor blockade (8-chloro-2,3,4,5-tetrahydro-3-methyl-5-phenyl-1H-3-benzazepine-7-ol (SCH 23390) and l-sulpiride, respectively) in the nucleus accumbens. However, the reduction was due to a change in the turning pattern. Blockade of muscarinic acetylcholine receptors in the nucleus accumbens did not change the contralateral turning induced by unilateral injection of dopamine receptor agonists into the nucleus accumbens. The results demonstrate that there is no functional interaction between the cholinergic and dopaminergic substrates involved, although blockade of the dopamine receptors elicited behavioural deficits that competed with the turning elicited by carbachol. The contralateral turning elicited by carbachol injection into the nucleus accumbens requires an intact dopamine activity at the level of dopamine D1and D2receptors in the ipsilateral, but not contralateral, ventrolateral striatum.