Liposomal Delivery of Purified Heat Shock Protein hsp70 Into Rat Pancreatic Islets as Protection Against Interleukin 1β–Induced Impaired β-Cell Function
Liposomal Delivery of Purified Heat Shock Protein hsp70 Into Rat Pancreatic Islets as Protection Against Interleukin 1β–Induced Impaired β-Cell Function
复制标题
将纯化的热休克蛋白 hsp70 脂质体递送至大鼠胰岛，以防止白细胞介素 1β 诱导的 β 细胞功能受损
作者：
B. Margulis;S. Sandler;D. Eizirik;N. Welsh;M. Welsh
近年来研究表明，长期暴露于白细胞介素1β（IL-1β）可诱导胰岛细胞产生热休克蛋白70（hsp 70）。目前尚不清楚这是否代表针对IL-1β的有害作用的细胞防御，或者hsp 70是否参与细胞因子的抑制作用。为了评估hsp 70在暴露于IL-1β的分离的胰岛中的作用，纯化hsp 70并通过脂质体技术将其导入分离的大鼠胰岛细胞中。根据免疫印迹分析，hsp 70的递送是有效的，但递送的hsp 70在16 h内消失。含Hsp 70的脂质体不影响蛋白质合成、胰岛素分泌或胰岛胰岛素mRNA含量。然而，当将hsp 70脂质体孵育的胰岛进一步暴露于IL-1β（25 U/ml）16 h时，这些胰岛响应于葡萄糖刺激而释放更多的胰岛素，并且比用对照脂质体孵育并随后暴露于细胞因子的胰岛含有更多的胰岛素mRNA。含牛血清白蛋白或卵清蛋白的脂质体没有观察到保护作用。结论：hsp 70对IL-1β诱导的胰岛β细胞功能损伤具有保护作用。
Recently it has been demonstrated that heat shock protein 70 (hsp70) is induced in pancreatic islet cells during prolonged exposure to interleukin 1β (IL-1β). It is unclear whether this represents a cellular defense against the noxious action of IL-1β or whether hsp70 is involved in the suppressive action of the cytokine. To assess the role for hsp70 in isolated islets exposed to IL-1β, hsp70 was purified and introduced into cells of isolated rat pancreatic islets via the liposome technique. Delivery of hsp70 was efficient according to immunoblot analysis, but delivered hsp70 disappeared within 16 h. Hsp70-containing liposomes did not affect protein synthesis, insulin secretion, or islet insulin mRNA content. However, when hsp70 liposome–incubated islets were further exposed to IL-1β (25 U/ml) for 16 h, these islets released more insulin in response to glucose stimulation and contained more insulin mRNA than islets incubated with control liposomes and subsequently exposed to the cytokine. No protective effect of liposomes containing bovine serum albumin or ovalbumin were observed. We conclude that hsp70 may protect against IL-1β–induced impairment of pancreatic β-cell function.
登录
查看更多内容
影响因子：
56.9
作者：
BECKMANN, RP;MIZZEN, LA;WELCH, WJ
通讯作者：
WELCH, WJ
影响因子：
56.9
作者：
BENDTZEN, K;MANDRUPPOULSEN, T;SVENSON, M
通讯作者：
SVENSON, M
白细胞介素 1 通过需要基因转录和 mRNA 翻译的过程抑制离体大鼠胰岛的胰岛素分泌。
DOI：
10.1172/jci114785
发表时间：
1990
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Hughes,JH;Colca,JR;Easom,RA;Turk,J;McDaniel,ML
通讯作者：
McDaniel,ML
细胞因子对胰岛β细胞的细胞毒性作用：类二十烷酸参与的证据。
影响因子：
4.8
作者：
Rabinovitch,A;Baquerizo,H;Sumoski,W
通讯作者：
Sumoski,W