Design of environmentally sensitive fluorescent 2'-deoxyguanosine containing arylethynyl moieties: distinction of thymine base by base-discriminating fluorescent (BDF) probe.
Design of environmentally sensitive fluorescent 2'-deoxyguanosine containing arylethynyl moieties: distinction of thymine base by base-discriminating fluorescent (BDF) probe.
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DOI：
10.1016/j.bmcl.2010.03.055
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发表时间：
2010-05
影响因子：
2.7
通讯作者：
Y. Shinohara;Katsuhiko Matsumoto;Kenji Kugenuma;T. Morii;Yoshio Saito;I. Saito
中科院分区：
文献类型：
--
作者：
Y. Shinohara;Katsuhiko Matsumoto;Kenji Kugenuma;T. Morii;Yoshio Saito;I. Saito
我们已经合成了各种取代的8-芳基乙基化2 ' -脱氧鸟苷衍生物。其中乙酰基取代脱氧鸟苷类似物4c表现出显著的溶剂依赖性荧光性质，即在非极性溶剂中荧光强烈，而在极性溶剂如甲醇中荧光极弱。通过使用溶剂荧光染色脱氧鸟苷类似物4c，我们开发了高度胸腺嘧啶(T)选择性荧光DNA探针，可以检测靶DNA中与T相反的4c，而不管侧翼序列如何。我们能够证明4c可以作为T特异性碱基鉴别荧光（BDF）核苷在均匀荧光分析。
We have synthesized various substituted 8-arylethynylated 2′-deoxyguanosine derivatives. Among them, acetyl substituted deoxyguanosine analogue 4c showed a remarkable solvent dependent fluorescence property, that is, an intense fluorescence in non-polar solvents but extremely weak fluorescence in polar solvents like methanol. By using solvatofluorochromic deoxyguanosine analogue 4c, we have developed highly thymine (T) selective fluorescent DNA probes that can sense T opposite 4c in a target DNA regardless of the flanking sequences. We were able to demonstrate that 4c can be used as a T specific base-discriminating fluorescent (BDF) nucleoside in homogeneous fluorescence assay.