Antimicrobial properties of resident coral mucus bacteria of Oculina patagonica
Antimicrobial properties of resident coral mucus bacteria of Oculina patagonica
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DOI：
10.1111/j.1574-6968.2009.01490.x
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发表时间：
2009-03-01
影响因子：
2.1
通讯作者：
Munn, Colin B.
中科院分区：
文献类型：
--
作者：
Nissimov, Jozef;Rosenberg, Eugene;Munn, Colin B.
对地中海珊瑚 Oculina patagonica 的珊瑚粘液微生物群落的抑制特性进行了检查。从珊瑚粘液中分离出 156 种不同的菌落形态类型，其中 9 种抑制希洛弧菌（Vibrio shiloi）的生长，这种弧菌先前被证明是该珊瑚的病原体。被鉴定为假交替单胞菌 (Pseudoalteromonas sp.) 的分离株。是 V. shiloi 最强的抑制剂。几种分离菌，特别是一种被鉴定为玫瑰杆菌属的菌株，也表现出对珊瑚病原体弧菌和海藻菌以及其他九种选定的革兰氏阳性和革兰氏阴性细菌的广谱作用。用 V. shiloi 接种先前建立的 Roseobacter 菌株生物膜，导致 3 小时内病原体活菌计数减少 5 个对数，而接种假交替单胞菌生物膜导致 3 小时后 V. shiloi 活力完全丧失。这些结果支持益生菌对与珊瑚全生物相关的微生物群落产生影响的概念。
The inhibitory properties of the microbial community of the coral mucus from the Mediterranean coral Oculina patagonica were examined. Out of 156 different colony morphotypes that were isolated from the coral mucus, nine inhibited the growth of Vibrio shiloi, a species previously shown to be a pathogen of this coral. An isolate identified as Pseudoalteromonas sp. was the strongest inhibitor of V. shiloi. Several isolates, especially one identified as Roseobacter sp., also showed a broad spectrum of action against the coral pathogens Vibrio coralliilyticus and Thallassomonas loyana, plus nine other selected Gram-positive and Gram-negative bacteria. Inoculation of a previously established biofilm of the Roseobacter strain with V. shiloi led to a 5-log reduction in the viable count of the pathogen within 3 h, while inoculation of a Pseudoalteromonas biofilm led to complete loss of viability of V. shiloi after 3 h. These results support the concept of a probiotic effect on microbial communities associated with the coral holobiont.