Aberrant expression of Arpin in human breast cancer and its clinical significance.
Aberrant expression of Arpin in human breast cancer and its clinical significance.
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Arpin在人乳腺癌中的异常表达及其临床意义
DOI：
10.1111/jcmm.12740
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发表时间：
2016-03
影响因子：
5.3
通讯作者：
Qu Z
中科院分区：
文献类型：
--
作者：
Liu X;Zhao B;Wang H;Wang Y;Niu M;Sun M;Zhao Y;Yao R;Qu Z
Arpin（Arp 2/3复合物抑制剂）是2013年发现的一种新蛋白，在细胞运动和迁移中起着关键作用。然而，Arpin在癌症中的确切作用尚不清楚。本研究探讨Arpin在乳腺癌中的表达，并评价其与乳腺癌患者临床病理特征和预后的相关性。对福尔马林固定、石蜡包埋的176例乳腺癌组织和43例正常乳腺组织进行Arpin蛋白的免疫组织化学（IHC），同时对来自乳腺癌患者的104对肿瘤和癌旁组织分别进行Arpin mRNA的qRT-PCR。在患者的回顾性队列分析中评估Arpin表达与临床病理特征和生存率的相关性。结果表明，Arpin蛋白在乳腺癌组织中的表达低于正常乳腺组织，而Arpin mRNA在乳腺癌组织中的表达也低于癌旁组织。单因素和多因素Logistic回归分析显示，176例乳腺癌患者中Arpin低表达与肿瘤、淋巴结和转移系统的分期密切相关，与腋窝淋巴结转移密切相关[比值比（OR）：3.242; 95%可信区间（CI）：1.526，6.888; P < 0.05]。此外，Arpin表达是无复发生存期的独立风险因素（HR：0.373; 95% CI：0.171，0.813; P < 0.05）。由于Arpin表达首次在人乳腺癌组织中用qRT-PCR和IHC检测，我们的结果表明Arpin下调可能有助于乳腺癌转移的开始和发展。因此，Arpin作为一种潜在的预测指标值得进一步研究。
Arpin (Arp2/3 complex inhibitor), a novel protein found in 2013, plays a pivotal role in cell motility and migration. However, the precise role of Arpin in cancer is unclear. This study investigated the expression of Arpin in breast cancer and evaluated its correlation with the characteristics of clinical pathology and prognosis of breast cancer patients. Immunohistochemistry (IHC) for Arpin protein was performed on formalin‐fixed, paraffin‐embedded 176 breast cancer tissues and 43 normal breast tissues while qRT‐PCR for Arpin mRNA with 104 paired tumour and paratumoural tissues from breast cancer patients respectively. The association of Arpin expression with clinical pathological features and survival was assessed in a retrospective cohort analysis of patients. The results showed that the expression of Arpin protein in cancer tissues was lower compared to that in normal breast and the expression of Arpin mRNA was also lower in cancer tissues than that in the matched paratumoural tissues. Among the 176 breast cancer patients, the lower expression of Arpin was significantly associated with advanced tumour, nodes and metastasis system stage, and the reduced Arpin expression was strongly associated with axillary lymph node metastasis using univariate and multivariate logistic regression analysis [odds ratio: 3.242; 95% confidence interval (CI): 1.526, 6.888; P < 0.05]. Furthermore, Arpin expression was an independent risk factor for recurrence‐free survival (HR: 0.373; 95% CI: 0.171, 0.813; P < 0.05). As Arpin expression was first examined in human breast cancer tissues with qRT‐PCR and IHC, our results suggest that Arpin downregulation may contribute to the initiation and development of breast cancer metastasis. Therefore, as a potential predictive marker, Arpin deserves future studies.