Dietary Arachidonic Acid Has a Time-Dependent Differential Impact on Adipogenesis Modulated via COX and LOX Pathways in Grass Carp Ctenopharyngodon idellus
Dietary Arachidonic Acid Has a Time-Dependent Differential Impact on Adipogenesis Modulated via COX and LOX Pathways in Grass Carp Ctenopharyngodon idellus
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DOI：
10.1007/s11745-016-4205-2
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发表时间：
2016-10
期刊：
影响因子：
1.9
通讯作者：
J. Tian;Cai-xia Lei;H. Ji;Liqiao Chen;Z. Du
中科院分区：
文献类型：
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作者：
J. Tian;Cai-xia Lei;H. Ji;Liqiao Chen;Z. Du
在本研究中，我们通过体内和体外模型探讨了花生四烯酸（ARA）在草鱼脂肪形成中的作用。为期8周的饲养试验采用三种等氮等能纯化饲料：无ARA、ARA和ARA +乙酰水杨酸[ASA，一种环氧化酶（COX）抑制剂]。分别于饲养4周和8周后取样。结果表明：ara饲料显著降低了鱼的腹腔脂肪指数（IPFI），脂肪细胞变小；饲喂8周后，ASA扭转了这种下降趋势。然而，在第4周，ARA组的IPFI和脂肪细胞大小更高，与ARA + ASA喂养的鱼相当。为了进一步研究ARA对草鱼脂肪细胞分化的影响，将草鱼融合前脂肪细胞与ARA孵育3天。体外实验表明ARA以剂量依赖的方式促进脂肪形成。用脂氧合酶（LOX）抑制剂去二氢愈创木酸预处理可以减弱ARA的促脂肪功能。然而，ARA处理8天后，脂肪细胞的脂质含量低于油酸处理的细胞，ASA可以抑制这一作用。由此揭示了ARA在草鱼脂肪形成中的双重功能。短期ARA治疗后，LOX途径可能在促进脂肪形成中发挥关键作用，而COX途径可能在长期治疗后抑制脂肪形成。
In this study, we explored the function of arachidonic acid (ARA) in adipogenesis in the grass carp (Ctenopharyngodon idellus) usingin vivoandin vitromodels. An 8-week feeding trial was performed using three isonitrogenous and isoenergetic purified diets: ARA-free, ARA, and ARA + acetylsalicylic acid [ASA, a cyclooxygenase (COX) inhibitor]. Fish were sampled after 4 and 8 weeks of feeding. Results showed that ARA-fed fish had a significantly lower intraperitoneal fat index (IPFI) and smaller adipocytes; these decreases were reversed by ASA after 8 weeks of feeding. Nevertheless, at week 4, the IPFI and adipocyte size were higher in the ARA group, and they were comparable to those of fish fed ARA + ASA. To further investigate the influence of ARA on adipocyte differentiation, confluent pre-adipocytes of grass carp were incubated with ARA for 3 days. Thisin vitroexperiment demonstrated that ARA promoted adipogenesis in a dose-dependent manner. Pre-treatment with the lipoxygenase (LOX) inhibitor nordihydroguaiaretic acid attenuated the pro-adipogenic function of ARA. However, after treatment with ARA for 8 days, adipocytes had a lower lipid content than cells treated with oleic acid, and ASA could suppress this effect. We thus revealed the dual function of ARA in adipogenesis in grass carp. The LOX pathway may play a key role in pro-adipogenesis after short-term treatment with ARA, whereas the COX pathway is possibly responsible for the inhibition of adipogenesis after long-term treatment.