Immature dendritic cells (CD1 1c+CD3-B220- cells) present in mouse peripheral blood
Immature dendritic cells (CD1 1c+CD3-B220- cells) present in mouse peripheral blood
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DOI：
10.1078/0171-2985-00186
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发表时间：
2002-10-01
期刊：
影响因子：
2.8
通讯作者：
Ikehara, S
中科院分区：
文献类型：
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作者：
Adachi, Y;Toki, J;Ikehara, S
众所周知，当外周血单个核细胞（PBMC）与GM-CSF一起培养时，树突状细胞（DC）从小鼠的外周血中发育。我们以前已经发现，不成熟的树突状细胞存在于血液中，甚至在人类。在本研究中，我们发现小鼠外周血中的CD 11 c（+）CD 3（-）B220（-）细胞是未成熟的DC。正常小鼠PBMCs中CD 11 c（+）CD 3（-）B220（-）细胞的百分比范围为0.5至2.5%。PBMC中的CD 11 c（+）CD 3（-）B220（-）细胞呈树突状，与脾脏中的CD 11 c（+）CD 3（-）B220（-）细胞形态相似，被认为是明确的DC。然而，它们实际上没有刺激同种异体T细胞增殖的能力，并且在脾脏中显示出比CD 11 c（+）CD 3（-）B220（-）细胞更低的MHC II类、B7-1和B7-2表达。当PBMCs中的CD 11 c（+）CD 3（-）B220（-）细胞与GM-CSF一起培养时，它们不仅显示出刺激同种异体T细胞增殖的有效能力，而且还显示出较高的MHC II类、B7-1和B7-2表达。此外，当静脉注射时，它们迁移到脾脏中。这些结果表明PBMC中的CD 11 c（+）CD 3（-）B220（-）细胞是未成熟的DC，并且它们迁移到脾脏中，在那里它们成熟。
It is well known that dendritic cells (DCs) are developed from the peripheral blood of mice when peripheral blood mononuclear cells (PBMCs) are cultured with GM-CSF. We have previously found that immature DCs are present in the blood even in humans. In the present study, we show that CD11c(+)CD3(-)B220(-) cells in the mouse peripheral blood are immature DCs. The percentage of CD11c(+)CD3(-)B220(-) cells in the (PBMCs) of normal mice ranges from 0.5 to 2.5%. The CD11c(+)CD3(-)B220(-) cells in the PBMCs show dendrites, similar in shape to the CD11c(+)CD3(-)B220(-) cells in the spleen, which are thought to be DCs definitely. However, they have practically no capacity to stimulate the proliferation of allogeneic T cells, and show a lower expression of MHC class II, B7-1 and B7-2 than CD11c(+)CD3(-)B220(-) cells in the spleen. When the CD11c(+)CD3(-)B220(-) cells in the PBMCs are cultured with GM-CSF, they show not only the potent ability to stimulate the proliferation of allogeneic T cells but also a higher expression of MHC class II, B7-1 and B7-2. Moreover, they migrate into the spleen when they are injected intravenously. These results suggest that CD11c(+)CD3(-)B220(-) cells in the PBMCs are immature DCs, and that they migrate into the spleen, where they mature.