Autocrine paracrine effects of overexpression of hepatocyte growth factor gene on growth of endothelial cells
Autocrine paracrine effects of overexpression of hepatocyte growth factor gene on growth of endothelial cells
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DOI：
10.1006/bbrc.1996.0440
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发表时间：
1996-03-27
影响因子：
3.1
通讯作者：
Ogihara, T
中科院分区：
文献类型：
--
作者：
Hayashi, S;Morishita, R;Ogihara, T
虽然肝细胞生长因子(HGF)是在血管细胞中合成的，但目前尚不清楚本地合成的HGF是否类似于外源添加的HGF。因此，我们将人HGF载体导入培养的细胞，并检测其对血管细胞生长的影响。转染HGF载体的内皮细胞(EC)合成和分泌高水平的HGF，且数量显著增加。将转导HGF载体的血管平滑肌细胞(VSMG)或EC的条件培养液加入未转染组，细胞数显著增加，此作用可被抗HGF抗体阻断。将转HGF基因的VSMG与EG进行GO培养，发现从VSMG或EC释放的HGF均能刺激EG的生长。这些结果表明，通过转染人HGF载体内源性产生的HGF对EG的生长具有自分泌和胰岛分泌的刺激作用，但对VSMC的生长无影响，提示局部HGF系统在心血管疾病中的作用。(C)1996年学术出版社。
Although hepatocyte growth factor (HGF) is synthesized in vascular cells, it is not known whether locally synthesized HGF acts similarly to exogenously added HGF. Therefore, we transfected cultured cells with human HGF vector and examined the effects on growth of vascular cells. Endothelial cells (EC) transfected with HGF vector synthesized and secreted high levels of HGF, and also showed significantly higher number. Addition of conditioned medium from vascular smooth muscle cells (VSMG) or EC transfected with HGF vector to nontransfected EG resulted in a significant increase in cell number, which was abolished by anti-HGF antibody. Go-culture of HGF-transfected VSMG with EG showed that HGF released from VSMG or EC stimulated EG growth. These results demonstrate that endogenously produced HGF by transfection of human HGF vector can exert autocrine and pancrine stimulatory effects on EG growth, but not VSMC growth, suggesting the role of local HGF system in cardiovascular disease. (C) 1996 Academic Press, Inc.