VITRIFICATION OF MOUSE ISLETS OF LANGERHANS - COMPARISON WITH A MORE CONVENTIONAL FREEZING METHOD
VITRIFICATION OF MOUSE ISLETS OF LANGERHANS - COMPARISON WITH A MORE CONVENTIONAL FREEZING METHOD
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DOI：
10.1016/0011-2240(87)90033-2
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发表时间：
1987-08-01
期刊：
影响因子：
2.7
通讯作者：
ZEILMAKER, GH
中科院分区：
文献类型：
--
作者：
JUTTE, NHPM;HEYSE, P;ZEILMAKER, GH
玻璃化冷冻保存胰岛的可能性进行了研究，使用冷冻保护剂的混合物，并与更传统的冷冻方法，使用Me2SO作为冷冻保护剂的结果进行了比较。将分离的小鼠胰岛分成三组：（1）培养6天的对照胰岛，（2）培养2天后通过玻璃化冷冻保存的胰岛，和（3）培养2天后在1.5M Me2SO中冷冻的胰岛。加温后，将来自组2和组3的胰岛培养4天。研究了这样处理的胰岛在2.5或25 mM葡萄糖存在下的胰岛素分泌、温热培养期间的存活率、形态学和逆转链脲佐菌素诱导的糖尿病的能力。从所有组的胰岛中的胰岛素分泌可以刺激的因素5或更多的葡萄糖浓度从2.5至25 mM的增加。在2.5 mM葡萄糖的存在下，胰岛素的分泌是相似的，在所有组的胰岛。在25 mM葡萄糖的存在下，胰岛素的分泌略低，但不显着低于冷冻保存的胰岛比对照noncryopreserved胰岛。两种方法冷冻保存后，胰岛在复温培养期间的存活率相当，两组胰岛均能降低链脲佐菌素糖尿病小鼠的血糖。我们得出结论，玻璃化方法冷冻保存的胰岛在体外和体内都是有功能的。这种方法快速、简单、便宜，因为避免了使用复杂的冷冻设备。
The possibility of cryopreservation of islets of Langerhans by vitrification using a mixture of cryoprotectants was investigated and the results were compared with a more conventional freezing method using Me2SO as cryoprotectant. Isolated mouse islets were divided into three groups:(1) control islets cultured for 6 days, (2) islets which were cryopreserved by vitrification after 2 days of culture, and (3) islets frozen in 1.5 M Me2SO after 2 days of culture. After warming, islets from groups 2 and 3 were cultured for 4 days. The thus treated islets were investigated with respect to insulin secretion in the presence of 2.5 or 25 mM glucose, survival during postwarming culture, morphology, and capability to reverse streptozotocin-induced diabetes. The insulin secretion in islets from all groups could be stimulated by a factor 5 or more by an increase in the concentration of glucose from 2.5 to 25 mM. The secretion of insulin in the presence of 2.5 mM glucose was similar in all groups of islets. The secretion of insulin in the presence of 25 mM glucose was slightly but not significantly lower in the cryopreserved islets than in the control noncryopreserved islets. The survival of islets during postwarming culture was comparable after cryopreservation with both methods, and islets from both groups could lower serum glucose in streptozotocin diabetic mice. We conclude that islets cryopreserved by the vitrification method are functional in vitro and in vivo. This method is quick, simple, and cheap because the use of complicated freezing equipment is avoided.