Prevalence and Contamination Patterns of Listeria monocytogenes in Flammulina velutipes Plants
Prevalence and Contamination Patterns of Listeria monocytogenes in Flammulina velutipes Plants
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金针菇植物中单增李斯特菌的流行情况和污染模式
DOI：
10.1089/fpd.2013.1727
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发表时间：
2014-08-01
影响因子：
2.8
通讯作者：
Yang, Xiaobing
中科院分区：
文献类型：
--
作者：
Chen, Moutong;Wu, Qingping;Yang, Xiaobing
摘要对金针菇生产企业进行抽样调查，了解单核细胞增多性李斯特菌的流行情况及污染源。在295份样品中，单核细胞增多性李斯特菌的患病率为18.6%；污染似乎来自菌丝刮刮机械，对产品和上游包装设备都造成了污染。55株单增李斯特菌中，I.1(1/2a-3a)和II.2(1/2b-3b-7)分别占65.5%和34.5%。此外，结晶紫染色和扫描电子显微镜显示，谱系I.1形成的生物膜明显比谱系II.2中的生物膜厚。利用肠杆菌重复基因间一致性-聚合酶链式反应和随机扩增多态性DNA对单核细胞增多性李斯特氏菌分离株进行了基因分型，结果表明，刮菌丝器表面可能是四种植物中三种单核细胞增多性李斯特氏菌污染的主要来源。这项研究是探索……的第一份报告。
Abstract Four mushroom (Flammulina velutipes) production plants were sampled to investigate the prevalence and contamination source of Listeria monocytogenes. Among 295 samples, the prevalence of L. monocytogenes was 18.6%; the contamination appeared to originate from the mycelium-scraping machinery, contaminating both the product and upstream packaging equipment. Of 55 L. monocytogenes isolates, lineages I.1 (1/2a-3a) and II.2 (1/2b-3b-7) accounted for 65.5% and 34.5%, respectively. In addition, lineage I.1 formed significantly thicker biofilms than those within lineage II.2, as determined by crystal violet staining and scanning electron microscopy. Genotype analyses of L. monocytogenes isolates using enterobacteria repetitive intergenic consensus-polymerase chain reaction, and random amplified polymorphic DNA revealed that the surfaces of mycelium-scraping machinery may serve as the main source of L. monocytogenes contamination in three of the four plants. This study was the first report to explore the ...