Effect of Rickettsia felis Strain Variation on Infection, Transmission, and Fitness in the Cat Flea (Siphonaptera: Pulicidae).
Effect of Rickettsia felis Strain Variation on Infection, Transmission, and Fitness in the Cat Flea (Siphonaptera: Pulicidae).
复制标题
猫立克次体菌株变异对猫蚤（Siphonaptera：Pulicidae）感染、传播和适应性的影响。
DOI：
10.1093/jme/tjx046
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发表时间：
2017
影响因子：
2.1
通讯作者：
Macaluso,KevinR
中科院分区：
文献类型：
--
作者：
Healy,SeanP;Brown,LisaD;Hagstrom,MelenaR;Foil,LaneD;Macaluso,KevinR
猫立克次体是一种由猫蚤猫栉首蚤（Bouché）（str. LSU）传播的人类病原体，也是孤雌生殖书虱嗜卷书虱（Liposcelis bostrychophila（Badonnel）（str. LSU-Lb）的专性共生体。研究了这两个菌株的遗传变异性对R.猫的宿主专化性和适合度以及由此可能导致的感染和传播动力学的差异。猫未知利用人工宿主系统，将猫蚤暴露于aR。费利斯海峡LSU-Lb感染的血餐，并监测感染，在7天的时间间隔为28天。实时定量PCR用于测定新暴露猫蚤中立克次体载量和感染密度，以及猫蚤之间的传播频率。persistentR.并对猫蚤F1代的猫蚤感染情况进行了评价。实验后7 d，76.7%的猫蚤成功获得R.费利斯海峡LSU-Lb. INR。费利斯海峡暴露于LSU-Lb的猫蚤，平均感染负荷（6.15 × 106）、感染密度（0.76）和感染率（91/114）均显著高于R.费利斯海峡感染负荷（3.09 × 106）、感染密度（0.68）、感染率（76/113）。一个持久的R.费利斯海峡LSU-Lb感染猫蚤成虫28 d，F1代未观察到雌雄比例畸变和垂直传播。而感染动力学不同，与较高的强度与R。费利斯海峡LSU-Lb，在F1后代中没有观察到明显的表型。
Rickettsia felisis a human pathogen transmitted by the cat flea,Ctenocephalides felis(Bouché) (str. LSU), as well as an obligate symbiont of the parthenogenic booklouseLiposcelis bostrychophila(Badonnel) (str. LSU-Lb). The influence of genetic variability in these two strains ofR. felison host specialization and fitness and possible resulting differences on infection and transmission kinetics inC. felisis unknown. Utilizing an artificial host system, cat fleas were exposed to aR. felisstr. LSU-Lb-infected bloodmeal and monitored for infection at 7-d intervals for 28 d. Quantitative real-time PCR was used to determine rickettsial load and infection density in newly exposed cat fleas, and transmission frequency between cat fleas. The effect of persistentR. felisinfection on cat flea F1progeny was also assessed. At 7 d postexposure 76.7% of the cat fleas successfully acquiredR. felisstr. LSU-Lb. InR. felisstr. LSU-Lb-exposed cat fleas, the mean infection load (6.15 × 106), infection density (0.76), and infection prevalence (91/114) were significantly greater thanR. felisstr. LSU infection load (3.09 × 106), infection density (0.68), and infection prevalence (76/113). A persistentR. felisstr. LSU-Lb infection was detected for 28 d in adult cat fleas but neither female:male ratio distortion nor vertical transmission was observed in F1progeny. While infection kinetics differed, with higher intensity associated withR. felisstr. LSU-Lb, no distinct phenotype was observed in the F1progeny.