Intracerebroventricular administration of chondroitinase ABC reduces acute edema after traumatic brain injury in mice.
Intracerebroventricular administration of chondroitinase ABC reduces acute edema after traumatic brain injury in mice.
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DOI：
10.1186/s13104-016-1968-8
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发表时间：
2016-03-12
影响因子：
1.8
通讯作者：
Morrison B 3rd
中科院分区：
文献类型：
--
作者：
Finan JD;Cho FS;Kernie SG;Morrison B 3rd
脑水肿是临床医生在重型颅脑损伤急性期面临的重大挑战。如果浮肿达到临界点，就会导致失控的颅内高压，如果不治疗，反过来又会导致严重的发病率或死亡。关于标准干预措施疗效的临床数据喜忧参半。这项研究的目的是验证一种新的治疗策略，以减少小鼠模型的创伤后脑水肿。之前的体外研究报告说，大脑膨胀是由于组成脑组织的基质中的大的、带负电荷的、不动的分子产生的静电和渗透力的耦合。软骨素酶ABC(ChABC)消化硫酸软骨素蛋白多糖，这是一种促成这种负电荷的分子。因此，我们在受控的小鼠皮质撞击脑损伤后，通过脑室(Icv)注射ChABC，并测量了相关的水肿变化。通过ChABC治疗，几乎一半的损伤所致的水肿被消除。脑室注射CHABC可能是治疗创伤性脑水肿急性期的一种新的有效方法。
Brain edema is a significant challenge facing clinicians managing severe traumatic brain injury (TBI) in the acute period. If edema reaches a critical point, it leads to runaway intracranial hypertension that, in turn, leads to severe morbidity or death if left untreated. Clinical data on the efficacy of standard interventions is mixed. The goal of this study was to validate a novel therapeutic strategy for reducing post-traumatic brain edema in a mouse model. Prior in vitro work reported that the brain swells due to coupled electrostatic and osmotic forces generated by large, negatively charged, immobile molecules in the matrix that comprises brain tissue. Chondroitinase ABC (ChABC) digests chondroitin sulfate proteoglycan, a molecule that contributes to this negative charge. Therefore, we administered ChABC by intracerebroventricular (ICV) injection after controlled cortical impact TBI in the mouse and measured associated changes in edema. Almost half of the edema induced by injury was eliminated by ChABC treatment. ICV administration of ChABC may be a novel and effective method of treating post-traumatic brain edema in the acute period.