Characterization of microsatellite markers in mandarin orange (Citrus reticulata Blanco)
Characterization of microsatellite markers in mandarin orange (Citrus reticulata Blanco)
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DOI：
10.1111/j.1471-8286.2007.01893.x
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发表时间：
2008-01-01
影响因子：
7.7
通讯作者：
Ollitrault, P.
中科院分区：
文献类型：
--
作者：
Froelicher, Y.;Dambier, D.;Ollitrault, P.
构建了一个富含二核苷酸的柑橘基因组文库。对101个阳性克隆进行了测序，并设计了引物。利用从越南采集的26-29个野生柑橘对这些基因座进行了变异水平的筛选。43个座位的等位基因数在2~18个之间，观察杂合度(H-O)在0.03~0.96之间，期望杂合度(H-E)在0.03~0.92之间。
A dinucleotide-enriched genomic library was obtained from mandarin orange (Citrus reticulata Blanco). A subset of 101 positive clones was sequenced and primers were designed. The loci were screened for levels of variation using 26-29 wild mandarin oranges collected in Vietnam. Forty-three loci were polymorphic with the number of alleles ranging from two to 18. The observed heterozygosity (H-O) and expected heterozygosity (H-E) were from 0.03 to 0.96 and from 0.03 to 0.92, respectively.