Dinucleotide relative abundance extremes: a genomic signature.
Dinucleotide relative abundance extremes: a genomic signature.
复制标题
DOI：
10.1016/s0168-9525(00)89076-9
复制
发表时间：
1995-07
期刊：
影响因子：
--
通讯作者：
Samuel Kariin;C. Burge
中科院分区：
文献类型：
--
作者：
Samuel Kariin;C. Burge
早期的生物化学实验证实，二核苷酸比值比或“一般设计”的集合是生物体DNA的显著稳定的性质，其在许多生物体中的蛋白质编码DNA、大量基因组DNA以及基因组DNA的不同复性率和密度梯度级分中基本上是相同的。对目前可用的基因组序列数据的分析扩展了这些早期的结果，表明基因组的不相交样本的一般设计基本上比来自其他生物体的序列彼此更相似，并且密切相关的生物体具有相似的一般设计。从这个角度来看，二核苷酸比值比（相对丰度）值的集合构成了每个DNA基因组的特征，其可以区分来自不同生物体的序列。二核苷酸比值比值似乎不仅反映了二核苷酸堆积能和碱基步骤构象偏好的化学性质，而且还反映了DNA修饰、复制和修复机制的物种特异性。
Early biochemical experiments established that the set of dinucleotide odds ratios or ‘general design' is a remarkably stable property of the DNA of an organism, which is essentially the same in protein-coding DNA, bulk genomic DNA, and in different renaturation rale and density gradient fractions of genomic DNA in many organisms. Analysis of currently available genomic sequence data has extended these earlier results, showing that the general designs of disjoint samples of a genome are substantially more similar to each other than to those of sequences from other organisms and that closely related organisms have similar general designs. From this perspective, the set of dinucleotide odds ratio (relative abundance) values constitute a signature of each DNA genome, which can discriminate between sequences from different organisms. Dinucleotide-odds ratio values appear to reflect not only the chemistry of dinucleotide stacking energies and base-step conformational preferences, but also the species-specific properties of DNA modification, replication and repair mechanisms.