Herbicide Resistance Endowed by Enhanced Rates of Herbicide Metabolism in Wild Oat (Avena spp.)
Herbicide Resistance Endowed by Enhanced Rates of Herbicide Metabolism in Wild Oat (Avena spp.)
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DOI：
10.1614/ws-d-12-00078.1
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发表时间：
2013-01-01
期刊：
影响因子：
2.5
通讯作者：
Powles, Stephen B.
中科院分区：
文献类型：
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作者：
Ahmad-Hamdani, M. S.;Yu, Qin;Powles, Stephen B.
研究了对乙酰辅酶A羧化酶（ACCase）抑制除草剂二氟草酮（difloflop -methyl）产生抗性的野生燕麦群体（R1）的生化基础。[C-14]-双氯膦的叶片吸收率和易位率在111和敏感(S)群体中是相同的。然而，与S相比，R1植株的植物毒性双氯草畏酸水平始终较低，而RI植株的无毒极性双氯草畏代谢物水平相对于S植株较高（高达1.7倍）。这些结果表明，在R1种群中，双氯草酸代谢率的提高是一种非靶位机制赋予了抗性。此外，RI中主要双氯膦代谢物的高效液相色谱洗脱谱与小麦相似，表明R1群体个体的抗性涉及一个由细胞色素P450单加氧酶介导的类似小麦的解毒系统。此外，使用非放射性超高效液相色谱质谱分析，在已知具有ACCase基因抗性突变的其他三个抗性野生燕麦群体（R2， R3和R4）的抗性个体中也观察到较低水平的组织双氯草酸。这些结果表明，单个野生燕麦植株可能存在一种或至少两种独立的抗性机制（目标位点的ACCase抗性突变和非目标位点的除草剂代谢率提高）。
The biochemical basis of resistance to the acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicide diflofop-methyl was investigated in a resistant wild oat population (R1), which does not exhibit a resistant ACCase. Rates of foliar uptake and translocation of [C-14]-diclofop were the same in the 111 vs. susceptible (S) populations. However, the level of phytotoxic diclofop acid was always found to be lower in the R1 vs. S plants, with a concomitant higher level (up to 1.7-fold) of nontoxic polar diclofop metabolites in RI relative to the S plants. These results indicate that a non-target-site-based mechanism of enhanced rate of diclofop acid metabolism confers resistance in population R1. Moreover, the high-performance liquid chromotography elution profile of the major diclofop metabolites in RI is similar to that of wheat, suggesting resistance in individuals of population R1 involves a wheat-like detoxification system mediated by cytochrome P450 monooxygenases. In addition, lower level of tissue diclofop acid was also observed using nonradioactive ultra-performance liquid chromatography mass spectrometry analysis in resistant individuals of three other resistant wild oat populations (R2, R3, and R4) known to posses ACCase gene resistance mutations. These results establish that either one or at least two independent resistance mechanisms (target-site ACCase resistance mutations and non target-site enhanced rates of herbicide metabolism) can be present in individual wild oat plants.