Enhanced expression of vimentin in motile prostate cell lines and in poorly differentiated and metastatic prostate carcinoma
Enhanced expression of vimentin in motile prostate cell lines and in poorly differentiated and metastatic prostate carcinoma
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DOI：
10.1002/pros.10088
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发表时间：
2002-09-01
期刊：
影响因子：
2.8
通讯作者：
Maitland, NJ
中科院分区：
文献类型：
--
作者：
Lang, SH;Hyde, C;Maitland, NJ
背景通过测量运动性和侵袭性分析一系列前列腺细胞系的转移潜力，并进一步将其与上皮分化标记物的表达相关联。使用体外测定法测量侵袭和运动性。细胞系和组织的免疫组织化学用于鉴定细胞角蛋白8和1、5、10、14、波形蛋白、前列腺特异性抗原、前列腺特异性膜抗原、雄激素受体、桥粒芯蛋白、E-钙粘蛋白、β 1整合素、CD 44、hmet、黏着斑蛋白和肌动蛋白TS的表达。波形蛋白的表达是唯一与运动相关的标志物，没有与侵袭相关的标志物。波形蛋白在运动性低的细胞（PNT 2-C2）中表达较低，在运动性高的细胞（P4 E6、PNT 1a、PC-3）中表达较高。Vimentin在高分化肿瘤中不表达，中分化肿瘤中有Vimentin阳性细胞（1/9骨扫描阴性，2/5骨扫描阳性），但大多数低分化肿瘤（4/11骨扫描阴性，9/14骨扫描阳性）和骨转移瘤（7/8）的肿瘤细胞中Vimentin高表达。运动性前列腺癌细胞系表达波形蛋白。在组织切片中，波形蛋白阳性肿瘤细胞的存在与低分化癌症和骨转移的存在呈正相关。
BACKGROUND. The metastatic potential of a series of prostate cell lines was analysed by measuring motility and invasiveness, and further correlated to the expression of epithelial differentiation markers.METHODS. Invasion and motility were measured using in vitro assays. Immunohistochemistry of cell lines and tissues was used to identify expression of cytokeratins 8 and 1, 5, 10, 14, vimentin, prostate specific antigen, prostate specific membrane antigen, androgen receptor, desmoglein, E-cadherin, beta1 integrin, CD44, hmet, vinculin and actin.RESULTS. Expression of vimentin was the only marker to correlate with motility, no markers correlated to invasion. Lower vimentin expression was observed in cells with low motility (PNT2-C2) and high expression in cells with high motility (P4E6, PNT1a, PC-3). Vimentin expression was not detected in well differentiated tumours, moderately differentiated tumors contained vimentin positive cells (1/9 bone scan negative, 2/5 bone scan positive), but the majority of poorly differentiated cancers (4/11 bone scan negative, 9/14 bone scan positive) and bone metastases (7/8) had high vimentin expression in tumor cells.CONCLUSIONS. Motile prostate cancer cell lines express vimentin. In tissue sections, the presence of vimentin positive tumour cells correlated positively to poorly differentiated cancers and the presence of bone metastases.