Orchestration of ethylene and gibberellin signals determines primary root elongation in rice
Orchestration of ethylene and gibberellin signals determines primary root elongation in rice
复制标题
乙烯和赤霉素信号的协调决定水稻初生根的伸长
DOI：
10.1093/plcell/koac008
复制
发表时间：
--
期刊：
影响因子：
--
通讯作者：
Huang Rongfeng
中科院分区：
文献类型：
--
作者：
Qin Hua;P;ey Bipin K.;Li Yuxiang;Huang Guoqiang;Wang Juan;Quan Ruidang;Zhou jiahao;Zhou Yun;Miao Yuchen;Zhang Dabing;Bennett Malcolm J.;Huang Rongfeng
禾谷类作物的初生根生长是早期建立幼苗和谷物产量的基础。水稻幼苗初生根在萌发后7-10 d快速生长，然后停止生长，但其生长机制尚不清楚。在这里，我们报告，乙烯和赤霉素（GA）的相互作用控制的协调发展的初生根在水稻幼苗。我们的分析推进的知识，主根生长维持较高的乙烯生产，降低生物活性GA含量。进一步的研究表明，乙烯信号转录因子ETHYLENE INSENSITIVE 3-LIKE 1（OsEIL 1）通过激活GA代谢基因GIBBERELLIN 2-OXIDASE 1（OsGA 2 ox 1）、OsGA 2 ox 2、OsGA 2 ox 3和OsGA 2 ox 5的表达，从而使GA活性失活，抑制根分生组织中的细胞增殖，最终逐渐抑制初生根的生长。OsGA 2 ox 3基因的突变减弱了乙烯诱导的GA失活，降低了根对乙烯的敏感性。基因分析表明OsGA 2 ox 3在OsEIL 1下游发挥功能。总之，我们确定了一个分子途径，乙烯影响水稻主根伸长过程中，并提供深入了解乙烯和GA信号的协调在根发育和幼苗建立。
Primary root growth in cereal crops is fundamental for early establishment of the seedling and grain yield. In young rice (Oryza sativa) seedlings, the primary root grows rapidly for 7–10 days after germination and then stops; however, the underlying mechanism determining primary root growth is unclear. Here, we report that the interplay of ethylene and gibberellin (GA) controls the orchestrated development of the primary root in young rice seedlings. Our analyses advance the knowledge that primary root growth is maintained by higher ethylene production, which lowers bioactive GA contents. Further investigations unraveled that ethylene signaling transcription factor ETHYLENE INSENSITIVE3-LIKE 1 (OsEIL1) activates the expression of the GA metabolism genesGIBBERELLIN 2-OXIDASE 1(OsGA2ox1),OsGA2ox2,OsGA2ox3, andOsGA2ox5, thereby deactivating GA activity, inhibiting cell proliferation in the root meristem, and ultimately gradually inhibiting primary root growth. Mutation inOsGA2ox3weakened ethylene-induced GA inactivation and reduced the ethylene sensitivity of the root. Genetic analysis revealed that OsGA2ox3 functions downstream of OsEIL1. Taken together, we identify a molecular pathway impacted by ethylene during primary root elongation in rice and provide insight into the coordination of ethylene and GA signals during root development and seedling establishment.