SEQUENCE AND GENOMIC ORGANIZATION OF NORWALK VIRUS
SEQUENCE AND GENOMIC ORGANIZATION OF NORWALK VIRUS
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DOI：
10.1006/viro.1993.1345
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发表时间：
1993-07-01
期刊：
影响因子：
3.7
通讯作者：
ESTES, MK
中科院分区：
文献类型：
--
作者：
JIANG, X;WANG, M;ESTES, MK
从人类志愿者的粪便中纯化的诺瓦克病毒获得重叠cdna文库（Jianget al.,1990,Science250, 1580-1583），用于获得病毒基因组的核苷酸序列。该序列共有7642个核苷酸（不包括3 ' poly(a)尾），碱基组成为48% G + c，序列中预测有3个开放阅读框（ORF）。根据与小核糖核酸病毒2C蛋白、3C蛋白酶和3D RNA依赖RNA聚合酶相似的序列鉴定，预测最长的ORF （ORF1，核苷酸（nt） 146至5359）编码非结构蛋白的多蛋白前体。ORF2 （nt 5346 ~ 6935）编码的多肽预计分子量为56,571 （56.6K，接近病毒衣壳蛋白的预期大小），它包含一个与小核糖核酸结构蛋白VP3相似的短序列区域。第三个潜在的ORF （nt 6938至7573）可以编码22.5K的小多肽。诺沃克病毒的基因组组织与猫的杯状病毒和兔出血性疾病病毒这两种杯状病毒的基因组有惊人的相似之处。诺瓦克病毒的形态、大小、极性和基因组组织表明它是冠状病毒科的一员。
A library of overlapping cDNAs obtained from Norwalk virus purified from stools of human volunteers (Jianget al.,1990,Science250, 1580-1583) was used to obtain the nucleotide sequence of the viral genome. The sequence has a total of 7642 nucleotides, excluding the 3′ poly(A) tail, and has a base composition of 48% G + C. Three open reading frames (ORF) are predicted in the sequence. The longest ORF (ORF1, nucleotides (nt) 146 to 5359) is predicted to encode a polyprotein precursor to nonstructural proteins based on identification of sequences similar to the picornavirus 2C protein, 3C protease, and 3D RNA-dependent RNA polymerase. ORF2 (nt 5346 to 6935) is predicted to encode a polypeptide with a predicted molecular weight of 56,571 (56.6K, close to the expected size of the viral capsid protein), and it contains a short region of sequence similarity to the picornavirus structural protein VP3. A third potential ORF (nt 6938 to 7573) could encode a small polypeptide of 22.5K. The genomic organization found in Norwalk virus shares striking similarities with the genome of two caliciviruses, the feline calicivirus and the rabbit hemorrhagio disease virus. The morphology, size, polarity, and genomic organization of the Norwalk virus indicate it is a member of the Caliciviridae family.