VIRAL AND CELLULAR FACTORS GOVERNING HAMSTER-CELL INFECTION BY MURINE AND GIBBON APE LEUKEMIA VIRUSES
VIRAL AND CELLULAR FACTORS GOVERNING HAMSTER-CELL INFECTION BY MURINE AND GIBBON APE LEUKEMIA VIRUSES
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DOI：
10.1128/jvi.65.11.5975-5982.1991
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发表时间：
1991-11-01
影响因子：
5.4
通讯作者：
EIDEN, MV
中科院分区：
文献类型：
--
作者：
WILSON, CA;EIDEN, MV
仓鼠细胞对大多数逆转录病毒的感染具有抵抗力，包括Moloney鼠白血病病毒(MoMLV)和长臂猿白血病病毒(GaLV)。我们构建了MoMLV-GalV杂交病毒粒子，以确定导致GalV和MoMLV无法感染仓鼠细胞的病毒和细胞决定因素。用MoMLV核心成分替代GalV核心成分，绕过了仓鼠细胞对GalV感染的抵抗力，表明仓鼠细胞具有GalV受体，但由于后穿透阻断而不能有效地感染这种灵长类逆转录病毒。相比之下，仓鼠细胞显然对MoMLV感染具有抵抗力，因为尽管它们携带MoMLV受体，但受体不起作用。用糖裂解抑制剂衣霉素处理CHO K1或BHK 21仓鼠细胞，可使这些细胞感染MoMLV。MoMLV-GalV杂合病毒粒子的构建可以有效地感染耐药细胞，从而可以鉴定出限制MoMLV和GalV感染仓鼠细胞的病毒和细胞因子。
Hamster cells are resistant to infection by most retroviruses, including Moloney murine leukemia virus (MoMLV) and gibbon ape leukemia viruses (GaLVs). We have constructed MoMLV-GaLV hybrid virions to identify viral and cellular determinants responsible for the inability of GaLV and MoMLV to infect hamster cells. The substitution of MoMLV core components for GaLV core components circumvents the resistance of hamster cells to infection by GaLV, demonstrating that hamster cells have receptors for GaLV but are not efficiently infected by this primate retrovirus because of a postpenetration block. In contrast, hamster cells are apparently resistant to MoMLV infection because although they bear a receptor for MoMLV, the receptor is nonfunctional. Treatment of CHO K1 or BHK 21 hamster cells with the glycoslyation inhibitor tunicamycin allows the cells to be infected by MoMLV. The construction of MoMLV-GaLV hybrid virions that can efficiently infect resistant cells has allowed the identification of viral and cellular factors responsible for restricting infection of hamster cells by MoMLV and GaLV.