3H-uridine uptake in human leukemia HL-60 cells exposed to extremely low frequency electromagnetic fields.
3H-uridine uptake in human leukemia HL-60 cells exposed to extremely low frequency electromagnetic fields.
复制标题
暴露于极低频电磁场的人类白血病 HL-60 细胞中的 3H-尿苷摄取。
DOI：
10.1016/0006-291x(92)91577-d
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
M. Miller
中科院分区：
文献类型：
--
作者：
M. Azadniv;M. Miller
人白血病HL-60细胞在体外暴露或假暴露于60 Hz的电场（3.40 mV/m）或磁场（10 G，1 μT）5小时，试图重复先前发表的报告（1）的结果，即类似暴露条件下[3 H]尿苷摄取增加。本实验的结果表明电场对尿苷掺入无处理效应。在阴性对照实验中证实了检测[3 H]尿苷摄取差异的能力。“阴性对照”实验表明，相对于4 °C，37 °C下细胞的3 H-尿苷摄取在统计学上显著增加。
Human leukemia HL-60 cellsin vitrowere exposed or sham-exposed to a 60 Hz electric (3.40 mV/m) or magnetic 10 G (1 μT) field for 5 hr in an attempt to replicate the results of a previously published report (1) of increased uptake of [3H] uridine in response to similar exposure conditions. The results of the present experiments indicated no treatment effect of the fields on the uridine incorporation. The ability to detect differences in [3H] uridine uptake were confirmed in a negative control experiment. A ‘negative control’ experiment demonstrated a statistically significant increase in3H-uridine uptake for cells at 37 °C relative to those at 4 °C.