V beta-dependent stimulation of bovine and human T cells by host-specific staphylococcal enterotoxins
V beta-dependent stimulation of bovine and human T cells by host-specific staphylococcal enterotoxins
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DOI：
10.1128/iai.65.10.4048-4054.1997
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发表时间：
1997-10-01
影响因子：
3.1
通讯作者：
Bohach, GA
中科院分区：
文献类型：
--
作者：
Deringer, JR;Ely, RJ;Bohach, GA
来自牛和绵羊物种的金黄色葡萄球菌分离物产生C型葡萄球菌肠毒素（SEC）的独特分子变体。SEC动物变体与SEC 1（一种人类相关SEC）具有大于98%的氨基酸序列同一性。根据其相应的宿主物种，两种SEC动物变体被命名为SEC牛和SECovine，我们以前表明，这些毒素在几种动物中诱导不同水平的T细胞刺激。本研究比较了这些密切相关的宿主特异性SEC变体刺激来自牛和人供体的携带V β的T细胞的能力。所有三种毒素都扩增了携带T细胞受体V β元件（huV β）的人T细胞3、12、13.2、14、15、17和20。然而，SEC 1导致hyV β 12的扩增大于SEC牛或SECovine。此外，牛T细胞以V β依赖的方式响应这些超抗原（SAg）而增殖。所有三种毒素都诱导携带先前测序的V β元件的牛T细胞增殖（boV β）来自牛T细胞克隆BTB13（boV β BTB13）、SEC1和SECovine也能够诱导携带boV β BTB35的牛T细胞增殖，而SECbovine不能刺激。这些密切相关的SEC变异体在T细胞增殖中表现出的种属特异性差异可能反映了S.金黄色葡萄球菌，可能是为了通过以宿主特异性方式操纵免疫系统来增加其宿主范围。
Staphylococcus aureus isolates from bovine and ovine species produce unique molecular variants of type C staphylococcal enterotoxin (SEC), The SEC animal variants have greater than 98% amino acid sequence identity with SEC1, a human-associated SEC, The two SEC animal variants have been designated SECbovine and SECovine according to their corresponding host species, We showed previously that these toxins induce quantitatively different levels of T-cell stimulation in several animal species. The present study compared the abilities of these closely related host-specific SEC variants to stimulate V beta-bearing T cells from bovine and human donors, All three toxins expanded human T cells bearing T-cell receptor V beta elements (huV beta) 3, 12, 13.2, 14, 15, 17, and 20, However, SEC1 resulted in greater expansion of hyV beta 12 than either SECbovine Or SECovine. In addition, bovine T cells proliferate in a V beta-dependent manner in response to these superantigens (SAgs), All three toxins induced the proliferation of bovine T cells bearing the previously sequenced V beta element (boV beta) from the bovine T-cell clone BTB13 (boV beta BTB13), SEC1 and SECovine also were able to induce proliferation of bovine T cells bearing boV beta BTB35, which SECbovine failed to stimulate. The species-specific differences in T-cell proliferation exhibited by these closely related SEC variants may reflect the evolutionary adaptation of S. aureus, presumably to increase its host range by the manipulation of the immune system in a host-specific manner.