miRNA expression analysis during normal zebrafish development and following inhibition of the hedgehog and notch signaling pathways
miRNA expression analysis during normal zebrafish development and following inhibition of the hedgehog and notch signaling pathways
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DOI：
10.1002/dvdy.21211
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发表时间：
2007-08-01
影响因子：
2.5
通讯作者：
Patton, James G.
中科院分区：
文献类型：
--
作者：
Thatcher, Elizabeth J.;Flynt, Alex S.;Patton, James G.
microRNAs （miRNAs）是一种小的（类似于22个核苷酸）非编码rna，在转录后水平调节基因表达，通常通过抑制翻译。据估计，编码这些小rna的基因约占动物基因组的2-3%，但可能调控大多数蛋白质编码基因，包括那些参与细胞规范和发育的基因。一个关键的问题是确定由microrna调节的靶mrna。作为识别潜在靶点的手段，我们设计了一个敏感的微阵列来分析斑马鱼十二个发育阶段的全球miRNA表达模式。此外，我们对使用Hedgehog和Notch信号通路小分子抑制剂处理的斑马鱼胚胎进行了阵列研究，以鉴定早期胚胎发生过程中控制关键发育通路的靶基因的差异表达mirna。
microRNAs (miRNAs) are small (similar to 22 nucleotide) non-coding RNAs that regulate gene expression at the post-transcriptional level, typically by inhibiting translation. The genes encoding these small RNAs are estimated to comprise approximately 2-3% of animal genomes yet potentially regulate a majority of protein-coding genes including those involved in cell specification and development. A key remaining question is to identify target mRNAs regulated by microRNAs. As a means to identify potential targets, we designed a sensitive microarray to analyze global miRNA expression patterns at twelve developmental stages in zebrafish. Further, we conducted arrays on zebrafish embryos treated with small molecule inhibitors of the Hedgehog and Notch signaling pathways to enable identification of differentially expressed miRNAs that target genes controlling key developmental pathways during early embryogenesis.