Peroxynitrite inhibition of Coxsackievirus infection by prevention of viral RNA entry.
Peroxynitrite inhibition of Coxsackievirus infection by prevention of viral RNA entry.
复制标题
DOI：
10.1073/pnas.0400518101
复制
发表时间：
2004-08
影响因子：
11.1
通讯作者：
E. Padalko;T. Ohnishi;K. Matsushita;H. Sun;K. Fox‐Talbot;C. Bao;W. Baldwin;C. Lowenstein
中科院分区：
文献类型：
--
作者：
E. Padalko;T. Ohnishi;K. Matsushita;H. Sun;K. Fox‐Talbot;C. Bao;W. Baldwin;C. Lowenstein
尽管过氧亚硝酸盐对宿主有害，但过氧亚硝酸盐的有益作用尚不清楚。我们探讨了过氧亚硝酸盐在宿主对柯萨奇病毒感染的免疫反应中的作用。过氧亚硝酸盐在体外抑制病毒复制，部分是通过抑制病毒 RNA 进入宿主细胞来实现的。硝基酪氨酸是过氧亚硝酸盐产生的标记物，它与受感染小鼠心脏中的病毒抗原共存，但对照小鼠则不然。硝基酪氨酸也与病毒多肽 VP1 共沉淀。胍基乙基二硫化物是一种过氧亚硝酸盐清除剂，可阻断过氧亚硝酸盐对体外病毒复制的抑制作用，并允许体内病毒复制增加。这些数据表明过氧亚硝酸盐是病毒免疫反应的内源效应物。
Although peroxynitrite is harmful to the host, the beneficial effects of peroxynitrite are less well understood. We explored the role of peroxynitrite in the host immune response to Coxsackievirus infection. Peroxynitrite inhibits viral replication in vitro, in part by inhibiting viral RNA entry into the host cell. Nitrotyrosine, a marker for peroxynitrite production, is colocalized with viral antigens in the hearts of infected mice but not control mice. Nitrotyrosine coprecipitates with the viral polypeptide VP1 as well. Guanidinoethyl disulfide, a scavenger of peroxynitrite, blocks peroxynitrite inhibition of viral replication in vitro and permits an increase in viral replication in vivo. These data suggest that peroxynitrite is an endogenous effector of the immune response to viruses.