Effect of Lactobacillus supplementation with and without arginine on liver damage and bacterial translocation in an acute liver injury model in the rat
Effect of Lactobacillus supplementation with and without arginine on liver damage and bacterial translocation in an acute liver injury model in the rat
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DOI：
10.1002/hep.510250325
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发表时间：
1997-03-01
期刊：
影响因子：
13.5
通讯作者：
Jeppsson, B
中科院分区：
文献类型：
--
作者：
Adawi, D;Kasravi, FB;Jeppsson, B
在肝炎、中毒性损伤或肝脏大手术后的急性肝功能衰竭中，肠道细菌移位增加。这可能解释了在这些情况下出现的一些感染性并发症。为了阐明其作用机制并寻找可能的预防措施，我们研究了精氨酸和益生菌(Lactobacillusspp.)直肠给药的效果。细菌易位和肝功能衰竭程度的关系。以SpragueDawley大鼠为实验对象，对五株不同的乳杆菌菌株(Lb.路透社R2LC，Lb.鼠李糖DSM 6594(=菌株271)，Lb.Platarum DSM 9843(=菌株299v)，Lb.发酵菌8704：3(=菌株245)和Lb.Reuri(=108株)每天直肠给药一次，连续8天，加和不加2%精氨酸。于第8天腹腔注射D-氨基半乳糖(1.1g/kg体重)诱导大鼠急性肝损伤(ALI)，分别于24、48h取材。通过门静脉和动脉血、肠系膜淋巴结和肝组织的细菌培养来评估细菌移位。测定血清中肝酶和胆红素水平。测定盲肠和结肠细菌载量，观察肝脏组织病理学变化。任何时候都没有死亡病例。与ALI对照组相比，添加精氨酸和不添加精氨酸的部分乳酸菌组的肝酶和胆红素降低。部分补充组的细菌易位发生率和易位细菌数显著降低。磅。车前草+精氨酸给药可显著降低盲肠和结肠中的肝酶释放量、肝细胞坏死和炎性细胞浸润、细菌移位以及肠杆菌数量。在ALI模型中，不同的乳杆菌菌株在精氨酸存在和不存在的情况下直肠给药可显著调节肝功能衰竭的程度，并减少细菌移位。磅。添加或不添加精氨酸的植物乳杆菌DSM 9843(=菌株299v)均优于其他乳杆菌菌株。单独给予精氨酸的有益效果表明，一氧化氮和多胺可能在这一过程中发挥作用，乳酸菌可能通过相同的机制或通过细菌拮抗和/或增强全身和肠道粘膜免疫来执行它们的作用。
In acute liver failure following hepatitis, toxic insults, or after major liver surgery, there is an increased bacterial translocation from the gut. This may explain some of the infectious complications seen in these conditions. To elucidate mechanisms and find possible preventive measures, we investigated the effect of rectal administration of arginine and probiotic bacteria (Lactobacillus spp.) on bacterial translocation and the extent of liver failure. Sprague-Dawley rats were used and five different Lactobacillus strains (Lb. reuteri R2LC, Lb. rhamnosus DSM 6594 (= strain 271), Lb. plantarum DSM 9843 (= strain 299v), Lb. fermentum 8704:3 (= strain 245), and Lb. reuteri (= strain 108) were administered rectally once daily for 8 days with and without 2% arginine. Acute liver injury (ALI) was induced on the eighth day by intraperitoneal injection of D-galactosamine (1.1 g/kg body weight), and samples were collected after 24 and 48 hours. Bacterial translocation was evaluated by bacterial culture hom portal and arterial blood, mesenteric lymph nodes, and liver tissue. Liver enzymes and bilirubin were evaluated in the serum. The bacterial load in the cecum and colon was determined and the liver histopathological changes were studied. There was no mortality at Any time. The liver enzymes and bilirubin decreased in some of the groups supplemented with lactobacilli with and without arginine compared with the ALI control group. The incidence of bacterial translocation and the number of the translocated bacteria decreased significantly in some of the supplemented groups. Lb. plantarum + arginine administration significantly reduced the level of the released liver enzymes, hepatocellular necrosis and inflammatory cell infiltration, bacterial translocation, and the number of Enterobacteriaceae in the cecum and colon. Rectal administration of different Lactobacillus strains with and without arginine in an ALI model significantly modulates the extent of the liver failure and reduces bacterial translocation. Lb. plantarum DSM 9843 (= strain 299v) with or without arginine seemed superior to the other Lactobacillus strains. The beneficial effect of arginine administration alone indicates a possible role of nitric oxide and polyamines in this process, and the lactobacilli may execute their action via the same mechanisms or via bacterial antagonism and/or enhancement of systemic and intestinal mucosal immunity.