Lysine acetylation participates in boar spermatozoa motility and acrosome status regulation under different glucose conditions
Lysine acetylation participates in boar spermatozoa motility and acrosome status regulation under different glucose conditions
复制标题
赖氨酸乙酰化参与不同葡萄糖条件下公猪精子运动和顶体状态的调节
DOI：
10.1016/j.theriogenology.2020.10.027
复制
发表时间：
2021-01-01
期刊：
影响因子：
2.8
通讯作者：
He, Bin
中科院分区：
文献类型：
--
作者：
Chen, Guo;Ren, Li;He, Bin
采用液态保存的猪精液进行高效的人工授精是养猪业的关键。葡萄糖是冷冻猪精液稀释液中使用最广泛的能量来源。然而，葡萄糖浓度和精子质量之间的关系尚未完全了解。在本研究中，猪精子被用作模型，以研究在生理温度（37摄氏度）和冷藏（17摄氏度）期间，不同的葡萄糖浓度对精子活力，线粒体活性和顶体完整性的影响。在37 ℃孵育3 h或17 ℃孵育3 d时，高糖组进行性运动精子的比例和线粒体活性显著低于低糖组，而在17 ℃孵育7 d时则无显著差异。赖氨酸乙酰化是一种可逆的翻译后修饰，在精子功能中起着至关重要的作用。结果表明，高糖组精子蛋白乙酰化水平高于低糖组。乙酰转移酶抑制剂（WM-1119）处理的精子中进行性运动和顶体完整精子的比例高于对照组。精子乙酰辅酶A的浓度，这是直接联系到乙酰化，是显着高于在高糖组比低葡萄糖组。总之，精子活力和顶体完整性可以通过改变延长液中葡萄糖的浓度来改变。高糖诱导的赖氨酸乙酰化参与了猪精子保存过程中精子活力和顶体完整性的调节。这些结果可为养猪业精子保存和人工授精提供参考。(C)2020爱思唯尔公司All rights reserved.
Efficient artificial insemination (AI) with liquid preserved boar semen is essential for the swine industry. Glucose is the most widely utilized energy source in refrigerated boar semen extenders. However, the relationship between glucose concentration and spermatozoa quality is not fully understood. In the present study, boar spermatozoa were used as a model to investigate the impact of different glucose concentrations on spermatozoa motility, mitochondrial activity, and acrosome integrity at physiological temperature (37 degrees C) and during refrigeration (17 degrees C). The proportion of progressively motile spermatozoa and mitochondrial activity in the high glucose group were significantly lower than in the low glucose group when incubated at 37 degrees C for 3 h or 17 degrees C for 3 d, but not at 17 degrees C for 7 d. Lysine acetylation is a reversible post-translational modification that plays a crucial role in spermatozoa function. Our results show that spermatozoa protein acetylation levels were higher in the high glucose group than in the low glucose group. The proportions of progressively motile and acrosome-intact spermatozoa were higher in acetyltransferase inhibitor (WM-1119)-treated spermatozoa than in the control. Spermatozoa acetyl-CoA concentration, which is directly linked to acetylation, was significantly higher in the high glucose group than in the low glucose group. Taken together, spermatozoa motility and acrosome integrity can be altered by changing the concentration of glucose in the extender. High glucose concentration-induced lysine acetylation participates in the regulation of boar spermatozoa motility and acrosome integrity during preservation. These results can provide insights into spermatozoa preservation and AI in the swine industry. (C) 2020 Elsevier Inc. All rights reserved.