Germline and somatic transformation of mating Tetrahymena thermophila by particle bombardment.
Germline and somatic transformation of mating Tetrahymena thermophila by particle bombardment.
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DOI：
10.1093/genetics/146.1.135
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发表时间：
1997-05
期刊：
影响因子：
3.3
通讯作者：
D. Cassidy-Hanley;J. Bowen;John H. Lee;E. Cole;Lynn Verplank;J. Gaertig;M. Gorovsky;P. Bruns
中科院分区：
文献类型：
--
作者：
D. Cassidy-Hanley;J. Bowen;John H. Lee;E. Cole;Lynn Verplank;J. Gaertig;M. Gorovsky;P. Bruns
交配的嗜热四膜虫在发育的不同时期受到核糖体 DNA 包被颗粒的轰击。回收了大核和微核转化体。转化的最佳发育阶段发生在微核减数分裂期间和大核原基形成期间。给出了引入质粒短暂保留的证据。遗传和分子测试证实，有性遗传转化与受体微核染色体同源位点的整合有关。
Mating Tetrahymena thermophila were bombarded with ribosomal DNA-coated particles at various times in development. Both macronuclear and micronuclear transformants were recovered. Optimal developmental stages for transformation occurred during meiosis for the micronucleus and during anlagen formation for the macronucleus. Evidence is given for transient retention of the introduced plasmid. Genetic and molecular tests confirmed that sexually heritable transformation was associated with integration at the homologous site in the recipient micronuclear chromosome.