A Selective NMR Method for Detecting Choline Containing Compounds in Liver Tissue: The 1H-14N HSQC Experiment
A Selective NMR Method for Detecting Choline Containing Compounds in Liver Tissue: The 1H-14N HSQC Experiment
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检测肝组织中含胆碱化合物的选择性 NMR 方法：1H-14N HSQC 实验
DOI：
10.1021/ja107745g
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发表时间：
2010-12-15
影响因子：
15
通讯作者：
Mao, Xi-an
中科院分区：
文献类型：
--
作者：
Mao, Jiezhen;Jiang, Ling;Mao, Xi-an
基于胆碱中质子与四极核N-14之间的J耦合，用小鼠肝脏证明了H-1-N-14 HSQC实验在组织上的可行性。选择性地观察到游离胆碱、磷酸胆碱和甘油磷酸胆碱H-1-N-14 HSQC信号，所有不需要的信号都被完全抑制。二维光谱中CH 2 O信号得到了很好的分辨，可用于定量分析。
The feasibility of a H-1-N-14 HSQC experiment on tissues is demonstrated with a mouse liver based on the J couplings between the protons and the quadrupolar nucleus N-14 in choline. Free choline, phosphocholine, and glycerolphosphocholine H-1-N-14 HSQC signals were selectively observed with all unwanted signals cleanly suppressed. The CH2O signals were well resolved in the two-dimensional spectrum, which can be used for quantitative analyses.