Isolation and characterisation of Pitef1 encoding the translation elongation factor EF-1α of the root endophyte Piriformospora indica
Isolation and characterisation of Pitef1 encoding the translation elongation factor EF-1α of the root endophyte Piriformospora indica
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DOI：
10.1055/s-2000-16647
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发表时间：
2000-11-01
期刊：
影响因子：
3.9
通讯作者：
Franken, P
中科院分区：
文献类型：
--
作者：
Bütehorn, B;Rhody, D;Franken, P
Piriformospora indica （Hymenomycetes, Basidiomycota）是一种新发现的内生菌，它与多种植物的根相互作用，对生物量产生积极影响。为了获得对籼稻进行分子研究的工具，对籼稻中编码翻译伸长因子EF-1 α的Pitef1进行了克隆和分析。基因组序列与cDNA序列比较发现，该基因编码区有7个内含子，5'非翻译区至少有1个内含子。通过Southern blot分析确定，Pitef1在基因组中仅作为一个拷贝存在。通过与玉米根系的交互作用，分析了该基因与菌丝发育和RNA积累的关系，结果表明该基因高表达。因此，Pitef1启动子应该是构建籼稻转化体系的载体的一个很好的工具。此外，基因Pitef1可能有助于估计植物发育过程中活性菌丝体的数量，并用于校正差异表达真菌基因的RNA积累分析。
Piriformospora indica (Hymenomycetes, Basidiomycota) is a newly described endophyte which interacts with the roots of a great variety of plants, showing a positive effect on biomass production. In order to obtain a tool for molecular studies on P. indica, Pitef1 encoding the translation elongation factor EF-1 alpha in P. indica has been cloned and analysed. Comparison of the genomic and cDNA sequence revealed the presence of seven introns in the coding part of the gene and at least one in the 5'untranslated region. Pitef1 is only present as one copy in the genome, as determined by Southern blot analysis. Interaction with roots of Zea mays in a time course experiment was analysed in relation to hyphal development and RNA accumulation, showing high expression of this gene. The Pitef1 promoter should therefore be a good tool to construct vectors for the development of a transformation system for P. indica. The gene Pitef1 might, in addition, be useful for estimating the amount of active mycelium during in planta development and for the calibration of RNA accumulation analyses of differentially expressed fungal genes.