Piezoelectric printing and probing of Lectin NanoProbeArrays for glycosylation analysis
Piezoelectric printing and probing of Lectin NanoProbeArrays for glycosylation analysis
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DOI：
10.1016/j.bbrc.2008.08.033
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发表时间：
2008-10-31
影响因子：
3.1
通讯作者：
Wiktor, Peter
中科院分区：
文献类型：
--
作者：
Nagaraj, Vinay J.;Eaton, Seron;Wiktor, Peter
聚糖作为疾病的生物标志物和治疗靶点具有巨大的潜力。然而，从临床样品中鉴定聚糖生物标志物的主要挑战是关键糖基化蛋白的丰度低。为了证明用纳升量的糖蛋白进行聚糖分析的潜力，我们开发了一种基于压电液体点胶的新技术（凝集素NanoProbeArray），用于凝集素阵列的非接触式印刷和探测。代替在凝集素阵列表面上淹没糖蛋白探针，如在常规的微阵列筛选中，压电打印机用于在阵列上的凝集素斑点上分配纳升的荧光标记的糖蛋白探针。作为概念验证。这里举例说明了凝集素纳米探针阵列精确鉴定和可靠区分胎球蛋白的密切相关的糖型的能力。与使用渐逝场扫描仪的凝集素阵列相当的灵敏度水平是沿着实现的，同时糖基化分析所需的探针量减少了几个数量级。爱思唯尔公司出版
Glycans have great potential as disease biomarkers and therapeutic targets. However, the major challenge for glycan biomarker identification from clinical samples is the low abundance of key glycosylated proteins. To demonstrate the potential for glycan analysis with nanoliter amounts of glycoprotein, we have developed a new technology (Lectin NanoProbeArray) based on piezoelectric liquid dispensing for non-contact printing and probing of a lectin array. Instead of flooding the glycoprotein probe on the lectin array surface, as in conventional microarray screening, a piezoelectric printer is used to dispense nano-liters of fluorescently labeled glycoprotein probe over the lectin spots on the array. As a proof-of-concept. the ability of Lectin NanoProbeArrays to precisely identify and reliably distinguish between the closely related glycoforrns of fetuin is illustrated here. Sensitivity levels comparable to lectin arrays that use evanescent-field scanners was achieved along with several orders of magnitude reduction in the amount of probe required for glycosylation analysis. Published by Elsevier Inc.