Influence of crosslinking on the mechanical behavior of 3D printed alginate scaffolds: Experimental and numerical approaches
Influence of crosslinking on the mechanical behavior of 3D printed alginate scaffolds: Experimental and numerical approaches
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DOI：
10.1016/j.jmbbm.2018.01.034
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发表时间：
2018-04-01
影响因子：
3.9
通讯作者：
Chen, Xiongbiao
中科院分区：
文献类型：
--
作者：
Naghieh, Saman;Karamooz-Ravari, Mohammad Reza;Chen, Xiongbiao
通过三维（3D）生物打印制造的组织支架在组织工程应用中引起了相当大的关注。由于水凝胶支架的机械性能应该与受损组织相匹配，因此已经研究了在3D生物打印期间改变各种参数以操纵所得支架的机械行为。使用阳离子溶液（如CaCl 2）交联支架对于调节机械性能也是重要的，但在文献中没有很好的记载。在这里，不同的交联剂的体积和交联时间的3D生物绘图藻酸盐支架的力学行为的影响进行了评估，使用实验和数值方法。通过压缩试验测量支架的弹性模量，建立有限元模型，并采用幂模型预测支架的力学行为。结果表明，交联时间和交联剂的体积都起着决定性的作用，在调制的三维生物绘图支架的力学性能。由于支架的机械性能会影响细胞反应，因此本研究的结果可以根据预期应用调节支架的弹性模量。
Tissue scaffolds fabricated by three-dimensional (3D) bioprinting are attracting considerable attention for tissue engineering applications. Because the mechanical properties of hydrogel scaffolds should match the damaged tissue, changing various parameters during 3D bioprinting has been studied to manipulate the mechanical behavior of the resulting scaffolds. Crosslinking scaffolds using a cation solution (such as CaCl2) is also important for regulating the mechanical properties, but has not been well documented in the literature. Here, the effect of varied crosslinking agent volume and crosslinking time on the mechanical behavior of 3D bioplotted alginate scaffolds was evaluated using both experimental and numerical methods. Compression tests were used to measure the elastic modulus of each scaffold, then a finite element model was developed and a power model used to predict scaffold mechanical behavior. Results showed that crosslinking time and volume of crosslinker both play a decisive role in modulating the mechanical properties of 3D bioplotted scaffolds. Because mechanical properties of scaffolds can affect cell response, the findings of this study can be implemented to modulate the elastic modulus of scaffolds according to the intended application.