Expression analysis suggests potential roles of microRNAs for phosphate and arbuscular mycorrhizal signaling in Solanum lycopersicum
Expression analysis suggests potential roles of microRNAs for phosphate and arbuscular mycorrhizal signaling in Solanum lycopersicum
复制标题
DOI：
10.1111/j.1399-3054.2009.01320.x
复制
发表时间：
2010-02-01
影响因子：
6.4
通讯作者：
Xu, Guohua
中科院分区：
文献类型：
--
作者：
Gu, Mian;Xu, Ke;Xu, Guohua
microRNAs（miRNAs）是一类在许多生物学过程中发挥重要作用的基因表达调控因子。近年来，有研究表明micoRNA参与了根瘤菌与植物共生关系的调控。然而，miRNAs在另一种类型的植物-微生物相互作用，丛枝菌根（AM）共生中的作用还没有记录。我们进行了微阵列筛选和聚（A）尾逆转录聚合酶链反应（RT-PCR）验证的miRNA表达在番茄（Solanum lycopersicum）在不同的磷酸盐（Pi）的可用性和AM共生条件下。在根中，miRNA 158、miRNA 862 - 3 p、miRNA 319、miRNA 394和miR 399在三种不同处理（Pi充足（+P）、Pi缺乏（-P）和AM共生（+M））下受到差异调节。在叶片中，多达14个miRNAs在Pi处理和AM共生的任一种或两种下上调或下调，其中miR 158、miR 319和miR 399在根和叶中均响应于处理。我们检测到叶片中的miR 395、miR779.1、miR 840和miR 867特异性地响应于AM共生，这与Pi的可用性无关，而叶片中的miR 398和根和叶中的miR 399是Pi饥饿诱导的。此外，根中的miR 158以及叶中的miR 837 - 3 p对Pi剥夺和AM定殖都有响应。相反，miR 862 - 3 p在根中响应于Pi营养，但不响应AM共生。此外，根中的miR 319和miR 394以及叶中的miR 158、miR 169 g *、miR 172、miR 172 b *、miR 319、miR 771和miR 775组成的miRNA组分别被磷饥饿上调和下调。这些数据表明，不同组的miRNA的表达改变是Pi饥饿诱导的反应和AM共生的重要组成部分。
MicroRNAs (miRNAs) have emerged as a class of gene expression regulators that play crucial roles in many biological processes. Recently, several reports have revealed that micoRNAs participate in regulation of symbiotic interaction between plants and nitrogen-fixing rhizobia bacteria. However, the role of miRNAs in another type of plant-microbe interaction, arbuscular mycorrhizal (AM) symbiosis, has not been documented. We carried out a microarray screen and poly(A)-tailed reverse transcriptase-polymerase chain reaction (RT-PCR) validation for miRNA expression in tomato (Solanum lycopersicum) under varying phosphate (Pi) availability and AM symbiosis conditions. In roots, miRNA158, miRNA862-3p, miRNA319, miRNA394 and miR399 were differentially regulated under three different treatments, Pi sufficient (+P ), Pi deficient (-P) and AM symbiosis (+M ). In leaves, up to 14 miRNAs were up- or down-regulated under either or both of the Pi treatments and AM symbiosis, of which miR158, miR319 and miR399 were responsive to the treatments in both roots and leaves. We detected that miR395, miR779.1, miR840 and miR867 in leaves were specifically responsive to AM symbiosis, which is independent of Pi availability, whereas miR398 in leaves and miR399 in both roots and leaves were Pi starvation induced. Furthermore, miR158 in roots as well as miR837-3p in leaves were responsive to both Pi deprivation and AM colonization. In contrast, miR862-3p in roots was responsive to Pi nutrition, but not to AM symbiosis. Moreover, the group of miRNA consisting miR319 and miR394 in roots and miR158, miR169g*, miR172, miR172b*, miR319, miR771 and miR775 in leaves were up- and down-regulated by Pi starvation, respectively. The data suggest that altered expression of distinct groups of miRNA is an essential component of Pi starvation-induced responses and AM symbiosis.