Totally synthetic crystalline ribonuclease A
Totally synthetic crystalline ribonuclease A
复制标题
全合成结晶核糖核酸酶A
DOI：
10.1002/bip.1981.360200910
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
N. Fujii
中科院分区：
文献类型：
--
作者：
H. Yajima;N. Fujii
通过应用三氟甲磺酸-茴香硫醚的新脱保护程序结合谷胱甘肽形成二硫化物的改良空气氧化程序，实现了牛胰腺核糖核酸酶（RNase）A的改进化学合成。在通过亲和层析纯化后，接着通过离子交换层析，获得具有完全酶活性的蛋白质，随后根据Kunitz从含水乙醇中结晶。因此，第一次以结晶形式获得具有完全RNase A活性的全合成酶。
Improved chemical synthesis of bovine pancreatic ribonuclease (RNase) A was achieved by applying a new deprotecting procedure with trifluoromethanesulfonic acid–thioanisole in combination with a modified air‐oxidation procedure with glutathione for the disulfide formation. After purifications by affinity chromatography, followed by ion‐exchange chromatography, a protein with the full enzymatic activity was obtained and subsequently crystallized from aqueous ethanol according to Kunitz. A totally synthetic enzyme with full RNase A activity was thus obtained in a crystalline form for the first time.