SUPPLEMENTARY ADENOVIRAL LEADER SEQUENCE AND ITS ROLE IN MESSENGER TRANSLATION
SUPPLEMENTARY ADENOVIRAL LEADER SEQUENCE AND ITS ROLE IN MESSENGER TRANSLATION
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DOI：
10.1016/0092-8674(78)90020-x
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发表时间：
1978-01-01
期刊：
影响因子：
64.5
通讯作者：
KELLER, W
中科院分区：
文献类型：
--
作者：
DUNN, AR;MATHEWS, MB;KELLER, W
在用腺病毒2-SV-40杂交体Ad 2 + ND 1 dp 2裂解感染的过程中，合成了病毒mRNA，其5“末端包括腺病毒2纤维基因的起始部分，其3”末端位于SV-40序列中。该杂交mRNA编码23- 24 K道尔顿的蛋白质。生物化学和EM异源双链分析表明，在感染后期，该mRNA的2种形式存在于[人宫颈癌HeLa]细胞的细胞质中。两种形式都含有存在于晚期腺病毒2 mRNA上的常规三联前导序列，但它们的不同之处在于存在或不存在由位于该基因上游2000个核苷酸的腺病毒2序列的180个核苷酸组成的第4前导序列。通过在氢氧化甲基汞-琼脂糖凝胶中分离含有总poly（A）的Ad 2 + ND 1 dp 2晚期细胞质RNA，分离了两种形式的杂合mRNA，并确定了第4前导序列的存在或缺失对信使在细胞中指导23- 24 K道尔顿蛋白合成的能力几乎没有或没有影响。来源于兔网织红细胞的自由翻译系统。
During the course of lytic infection with the adenovirus 2-SV-40 hybrid Ad2+ND1 dp2, a viral mRNA is synthesized whose 5'' end includes the start of the adenovirus 2 fiber gene and whose 3'' end lies in SV-40 sequences. This hybrid mRNA codes for a protein(s) of 23-24K daltons. Biochemical and EM heteroduplex analyses reveal that 2 forms of this mRNA exist in the cytoplasm of [human cervical carcinoma HeLa] cells at late times in infection. Both forms contain the conventional tripartite leader present on late adenovirus 2 mRNA, but they differ in the presence or absence of a 4th leader sequences consisting of 180 nucleotides of adenovirus 2 sequences located 2000 nucleotides upstream from the gene. By fractionation of total poly(A)-containing Ad2+ND1 dp2 late cytoplasmic RNA in methyl mercuric hydroxide-agarose gels, both forms of the hybrid mRNA were isolated and it was established that the presence or absence of the 4th leader sequence has little or no influence on the ability of the messenger to direct the synthesis of the 23-24K dalton protein(s) in a cell-free translation system derived from rabbit reticulocytes.