Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro
Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro
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DOI：
10.1007/s13758-012-0029-7
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发表时间：
2012-12-01
期刊：
影响因子：
2.1
通讯作者：
Jiang, Xingyu
中科院分区：
文献类型：
--
作者：
Sun, Yi;Huang, Zhuo;Jiang, Xingyu
通过对多种大分子表面涂膜剂的定量比较，我们发现表面涂覆剂对原代培养的海马神经元的黏附和形态发生有很强的调控作用，并在体外起到了胞体聚集和轴突丛生的开关作用。神经元在聚赖氨酸涂层表面的黏附动力学比在层粘连蛋白和Matrigel涂层表面的黏附动力学快得多，而且黏附在聚赖氨酸表面的分布更均匀。另一方面，Matrigel和Laminin比多聚赖氨酸更能促进神经发生。最终，在自组装单分子层的Matrigel涂层表面，神经元倾向于经历体细胞聚集和轴突丛生。通过用大脑星形胶质细胞代替包被蛋白，并通过自组装单层膜、微流体和微接触打印将神经元图案化在星形胶质细胞上，我们发现星形胶质细胞促进胞体黏附，星形胶质细胞突起引导突起。在那里，星形胶质细胞可能是体外工程神经元网络的一种多功能底物。此外，对不同涂层上细胞反应的定量测量将为神经生物界选择最合适的涂层策略提供有价值的信息。
By quantitatively comparing a variety of macromolecular surface coating agents, we discovered that surface coating strongly modulates the adhesion and morphogenesis of primary hippocampal neurons and serves as a switch of somata clustering and neurite fasciculation in vitro. The kinetics of neuronal adhesion on poly-lysine-coated surfaces is much faster than that on laminin and Matrigel-coated surfaces, and the distribution of adhesion is more homogenous on poly-lysine. Matrigel and laminin, on the other hand, facilitate neuritogenesis more than polylysine does. Eventually, on Matrigel-coated surfaces of self-assembled monolayers, neurons tend to undergo somata clustering and neurite fasciculation. By replacing coating proteins with cerebral astrocytes, and patterning neurons on astrocytes through self-assembled monolayers, microfluidics and micro-contact printing, we found that astrocyte promotes soma adhesion and astrocyte processes guide neurites. There, astrocytes could be a versatile substrate in engineering neuronal networks in vitro. Besides, quantitative measurements of cellular responses on various coatings would be valuable information for the neurobiology community in the choice of the most appropriate coating strategy.