The tomato photomorphogenetic mutant, aurea, is deficient in phytochromobilin synthase for phytochrome chromophore biosynthesis.
The tomato photomorphogenetic mutant, aurea, is deficient in phytochromobilin synthase for phytochrome chromophore biosynthesis.
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DOI：
10.1093/pcp/pci062
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发表时间：
2005-04
影响因子：
4.9
通讯作者：
T. Muramoto;C. Kami;H. Kataoka;N. Iwata;P. Linley;Keiko Mukougawa;A. Yokota;T. Kohchi
中科院分区：
文献类型：
--
作者：
T. Muramoto;C. Kami;H. Kataoka;N. Iwata;P. Linley;Keiko Mukougawa;A. Yokota;T. Kohchi
番茄突变体是一类光敏色素缺失突变体，广泛应用于光形态发生和光生物学研究。通过表达序列标签（EST）筛选序列数据库，我们发现了一个番茄基因，该基因编码与拟南芥HY2同源的光敏色素合成酶，催化光敏色素生色团生物合成的最后一步。在大肠杆菌中表达的番茄蛋白具有光敏色素合成酶活性。所有突变体的相应位点都存在核苷酸替换、缺失或DNA重排。这些结果表明，aurea是番茄光敏色素合成酶的突变体。我们还讨论了在胆色素还原酶家族的phytochromobilin酶的系统发育分析。
The aurea mutants of tomato have been widely used as phytochrome-deficient mutants for photomorphogenetic and photobiological studies. By expressed sequence tag (EST)-based screening of sequence databases, we found a tomato gene that encodes a protein homologous to Arabidopsis HY2 for phytochromobilin synthase catalyzing the last step of phytochrome chromophore biosynthesis. The tomato protein expressed in Escherichia coli showed phytochromobilin synthase activity. The corresponding loci in all aurea mutants tested have nucleotide substitutions, deletions or DNA rearrangements. These results indicate that aurea is a mutant of phytochromobilin synthase in tomato. We also discuss a phylogenetic analysis of phytochromobilin synthases in the bilin reductase family.