THE HUMAN GROWTH-HORMONE GENE FAMILY - STRUCTURE AND EVOLUTION OF THE CHROMOSOMAL LOCUS
THE HUMAN GROWTH-HORMONE GENE FAMILY - STRUCTURE AND EVOLUTION OF THE CHROMOSOMAL LOCUS
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DOI：
10.1093/nar/11.12.3939
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发表时间：
1983-01-01
影响因子：
14.9
通讯作者：
GELINAS, RE
中科院分区：
文献类型：
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作者：
BARSH, GS;SEEBURG, PH;GELINAS, RE
人类生长激素基因簇的结构已经确定在DNA的一个78千碱基区域的研究两个重叠的宇宙。两种生长激素基因与三种绒毛膜促生长激素基因穿插在一起，它们的转录方向相同。其中一种生长激素基因位于垂体的活性染色质构象中，至少一种绒毛膜促生长激素基因位于胎盘的活性染色质构象中。这两组基因在它们的5 ‘侧翼和编码序列上是高度同源的，但在它们的3 ’侧翼区域却是不同的，这就提出了一个悖论，即在结构和侧翼序列上如此相似的基因如何被如此不同的调控。对基因序列的分析和至少三种不同类型的重复单元的鉴定表明，该基因集群的进化相当近，基因复制的机制涉及Alu家族中间重复元件之间同源但不平等的交换。
The structure of the human growth hormone gene cluster has been determined over a 78 kilobase region of DNA by the study of two overlapping cosmids. There are two growth hormone genes interspersed with three chorionic somatomammotropin genes, all in the same transcriptional orientation. One of the growth hormone genes lies in an active chromatin conformation in the pituitary and at least one of the chorionic somatomammotropin genes lies in an active chromatin conformation in the placenta. The two groups of genes are highly homologous throughout their 5′ flanking and coding sequences, but diverge in their 3′ flanking regions which raises the paradox of how genes so similar in structural and flanking sequences can be so differentially regulated. Analysis of the sequences of the genes and identification of at least three different classes of duplication units interspersed throughout the five gene cluster suggests that the cluster evolved quite recently and that the mechanism of gene duplication involved homologous but unequal exchange between middle repetitive elements of the Alu family.