Initial observations on the isolated retinal pigment epithelium-choroid of the cat.
Initial observations on the isolated retinal pigment epithelium-choroid of the cat.
复制标题
对猫分离的视网膜色素上皮-脉络膜的初步观察。
DOI：
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发表时间：
1978
影响因子：
4.4
通讯作者：
W. Stern
中科院分区：
文献类型：
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作者：
R. H. Steinberg;S. Miller;W. Stern
为从神经性视网膜分离研究哺乳动物视网膜色素上皮(RPE)脉络膜，建立了一种哺乳动物视网膜色素上皮(RPE)-脉络膜的体外制备方法。这些初步实验的目的是评估组织的电学特性和RPE根尖膜的离子特性。他们的设计是为了使结果直接与之前在更广泛研究的冷血制剂牛蛙上获得的结果相媲美。最好的CAT组织的电学特性与常规获得的牛蛙相似，但平均CAT顶膜电位比青蛙的去极化多20 mV。猫的顶膜对钾的敏感性与蛙的相似，但对碳酸氢盐的敏感性相对较小，对钠的敏感性相对较大。像青蛙一样，猫的顶膜上也有一种哇巴因敏感机制，直接对膜电位做出贡献。哺乳动物盐溶液中较高的钾浓度和顶膜的钠电导是导致猫顶膜电位较低的两个因素。
An in vitro preparation of a mammalian retinal pigment epithelium (RPE)-choroid was developed, that of the cat, in order to study the RPE in isolation from the neural retina. The purpose of these initial experiments was to evaluate the electrical characteristics of the tissue and the ionic properties of the RPE apical membrane. They were designed so that results would be directly comparable to those previously obtained on a more extensively studied cold-blooded preparation, the bullfrog. The electrical characteristics of the best cat tissues were similar to those routinely obtained in bullfrog, whereas the average cat apical membrane potential was 20 mV more depolarized than that of frog. The apical membrane of cat resembled the frog's in having a large sensitivity to potassium, but it had a relatively smaller sensitivity to bicarbonate and a relatively larger sensitivity to sodium. The cat, like the frog, also had a ouabain-sensitive mechanism on its apical membrane that directly contributed to membrane potential. Two factors contributing to the lower apical membrane potential in cat were the higher potassium concentration of the mammalian salt solution and the sodium conductance of the apical membrane.