MICROFILAMENT DISTRIBUTION IN MAIZE MEIOTIC MUTANTS CORRELATES WITH MICROTUBULE ORGANIZATION
MICROFILAMENT DISTRIBUTION IN MAIZE MEIOTIC MUTANTS CORRELATES WITH MICROTUBULE ORGANIZATION
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DOI：
10.1105/tpc.3.6.637
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发表时间：
1991-06-01
期刊：
影响因子：
11.6
通讯作者：
CANDE, WZ
中科院分区：
文献类型：
--
作者：
STAIGER, CJ;CANDE, WZ
微管和微丝通常在植物中共分布；它们假定的相互作用可以用药物进行测试，尽管并不总是清楚这些药物是否没有副作用。在这项研究中，我们利用减数分裂细胞分裂缺陷突变体，以非侵入性的方式研究这两种细胞骨架元件之间的关系。用罗丹明-phalloidin染色未固定的渗透细胞，观察玉米减数分裂过程中微丝分布的时空变化。在野生型小孢子细胞中，从核放射出的微管阵列在纺锤体形成过程中消失，并在末期恢复。这个结果不同于复杂的细胞质微丝阵列，存在于所有阶段，包括核分裂和细胞质分裂。在分裂过程中，纺锤体和片质体中也出现了第二类微丝。为了分析这种微管和微丝的明显关联，我们研究了几种已知的减数分裂突变体，它们的微管阵列具有特定阶段的破坏。两种改变减数分裂纺锤体数量或形式的突变也导致了f -肌动蛋白的戏剧性重组。相反，非纺锤体细胞质微管的重排不会导致f -肌动蛋白分布的变化。这些结果表明，在高等植物减数分裂过程中，微管和微丝以细胞周期特异性和位点特异性的方式相互作用。
Microtubules and microfilaments often codistribute in plants; their presumed interaction can be tested with drugs although it is not always clear that these are without side effects. In this study, we exploited mutants defective in meiotic cell division to investigate in a noninvasive way the relationship between the two cytoskeletal elements. By staining unfixed, permeabilized cells with rhodamine-phalloidin, spatial and temporal changes in microfilament distribution during maize meiosis were examined. In wild-type microsporocytes, a microtubule array that radiates from the nucleus disappeared during spindle formation and returned at late telophase. This result differed from the complex cytoplasmic microfilament array that is present at all stages, including karyokinesis and cytokinesis. During division, a second class of microfilaments also was observed in the spindle and phragmoplast. To analyze this apparent association of microtubules and microfilaments, we examined several meiotic mutants known to have stage-specific disruptions in their microtubule arrays. Two mutations that altered the number or form of meiotic spindles also led to a dramatic reorganization of F-actin. In contrast, rearrangement of nonspindle, cytoplasmic microtubules did not lead to concomitant changes in F-actin distribution. These results suggested that microtubules and microfilaments interact in a cell cycle-specific and site-specific fashion during higher plant meiosis.