Filamentation Regulatory Pathways Control Adhesion-Dependent Surface Responses in Yeast
Filamentation Regulatory Pathways Control Adhesion-Dependent Surface Responses in Yeast
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DOI：
10.1534/genetics.119.302004
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发表时间：
2019-07-01
期刊：
影响因子：
3.3
通讯作者：
Cullen, Paul J.
中科院分区：
文献类型：
--
作者：
Chow, Jacky;Starr, Izzy;Cullen, Paul J.
信号通路通过对靶基因的转录调控来调节生物反应。在酵母中，多种信号通路控制丝状生长，这是一种发生在包括真菌病原体在内的许多物种中的形态发生反应。在这里，我们研究的作用，控制丝状生长的信号通路调节粘附依赖的表面反应，包括垫的形成和菌落图案。表达谱分析和突变体表型分析表明，调节丝状生长的主要途径[丝状生长MAPK（fMAPK）、RAS、逆行（RTG）、RIM101、RPD3、ELP、SNF1和PHO85]也调节垫形成和集落模式。染色质重塑复合物，佐贺，也调节这些反应。我们还表明，RAS和RTG途径共调节一组共同的靶基因，佐贺调节已知由fMAPK，RAS和RTG途径控制的靶基因。表面生长特异性靶点的分析确定了对低氧、高温和干燥胁迫作出反应的基因。我们还探讨了为什么细胞在菌落中进行粘附接触的问题。由共调节靶点和粘附分子介导的细胞粘附接触阻止宏观捕食者进入菌落，并影响菌落温度调节。确定新的监管机构（例如，佐贺），和酵母表面生长的目标可以提供在表面生长和粘附有助于毒力的设置真菌发病机制的见解。
Signaling pathways can regulate biological responses by the transcriptional regulation of target genes. In yeast, multiple signaling pathways control filamentous growth, a morphogenetic response that occurs in many species including fungal pathogens. Here, we examine the role of signaling pathways that control filamentous growth in regulating adhesion-dependent surface responses, including mat formation and colony patterning. Expression profiling and mutant phenotype analysis showed that the major pathways that regulate filamentous growth [filamentous growth MAPK (fMAPK), RAS, retrograde (RTG), RIM101, RPD3, ELP, SNF1, and PHO85] also regulated mat formation and colony patterning. The chromatin remodeling complex, SAGA, also regulated these responses. We also show that the RAS and RTG pathways coregulated a common set of target genes, and that SAGA regulated target genes known to be controlled by the fMAPK, RAS, and RTG pathways. Analysis of surface growth-specific targets identified genes that respond to low oxygen, high temperature, and desiccation stresses. We also explore the question of why cells make adhesive contacts in colonies. Cell adhesion contacts mediated by the coregulated target and adhesion molecule, , deterred entry into colonies by macroscopic predators and impacted colony temperature regulation. The identification of new regulators (e.g., SAGA), and targets of surface growth in yeast may provide insights into fungal pathogenesis in settings where surface growth and adhesion contributes to virulence.