Syringic acid (4-hydroxy-3,5-dimethoxybenzoic acid) inhibits adipogenesis and promotes lipolysis in 3T3-L1 adipocytes
Syringic acid (4-hydroxy-3,5-dimethoxybenzoic acid) inhibits adipogenesis and promotes lipolysis in 3T3-L1 adipocytes
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DOI：
10.1080/14786419.2019.1573820
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发表时间：
2020-12-01
影响因子：
2.2
通讯作者：
Arockiasamy, Sumathy
中科院分区：
文献类型：
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作者：
John, Cordelia Mano;Arockiasamy, Sumathy
肥胖的增加与肥胖和肥胖相关的并发症有关。丁香酸(SA)是一种促进氧化、聚合和缩合反应的酚酸，可作为一种有效的抗脂药物发挥作用。本研究检测SA是否通过抑制分化和控制脂质积累来抑制3T3-L1前脂肪细胞的成脂。油红O染色显示，SA处理10天后，3T3-L1前脂肪细胞向成熟脂肪细胞的分化受到抑制，且呈浓度依赖关系。SA对成脂分化的抑制主要是通过抑制分化早期有丝分裂克隆的扩增来实现的。用SA处理脂肪细胞减少了甘油三酯的产生(脂肪生成)，增加了甘油的释放(脂肪分解)。在体外培养的脂肪细胞中加入SA可抑制ROS的产生。这些结果表明，SA抑制3T3-L1前脂肪细胞的成脂，减少脂肪堆积，抑制ROS堆积，首次表明SA在体外具有抗肥胖作用。
Increase in adiposity is associated with obesity and obesity-related complications. Syringic acid (SA), a phenolic acid facilitates oxidation, polymerization, and condensation reactions, may function as an effective anti-adipogenic agent. This study examined whether SA inhibits adipogenesis in 3T3-L1 preadipocytes by inhibiting differentiation and controlling lipid accumulation. Ten days of SA treatment suppressed differentiation of 3T3-L1 preadipocyte to mature adipocytes in a concentration-dependent manner as determined by Oil Red O staining. Suppression of adipogenic differentiation by SA was primarily from inhibition of mitotic clonal expansion during the early stages of differentiation. Treatment of adipocytes with SA reduced triglyceride production (lipogenesis) and increased glycerol release (lipolysis). The addition of SA to the adipocytes attenuated in vitro ROS production. These results indicate that SA inhibits the adipogenesis of 3T3-L1 preadipocytes, reduces lipid accumulation, and attenuates ROS accumulation, suggesting for the first time that SA has an anti-obesity effect in vitro.