Sequence conservation of glycerophosphodiester phosphodiesterase among Treponema pallidum strains
Sequence conservation of glycerophosphodiester phosphodiesterase among Treponema pallidum strains
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DOI：
10.1128/iai.67.6.3168-3170.1999
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发表时间：
1999-06-01
影响因子：
3.1
通讯作者：
Van Voorhis, WC
中科院分区：
文献类型：
--
作者：
Cameron, CE;Castro, C;Van Voorhis, WC
以往的研究表明，接种梅毒螺旋体（Treponema pallidum subsp.苍白球甘油磷酸二酯磷酸二酯酶显著保护兔免受随后的密螺旋体攻击。在这份报告中，我们表明，甘油磷酸二酯磷酸二酯酶的氨基酸序列是保守的12株共5个致病性密螺旋体。这种免疫保护性抗原的不变性质使其成为包含在针对T的通用亚单位疫苗中的有吸引力的候选物。苍白球感染此外，这些研究显示在非T中一致观察到的gpd开放阅读框的位置579处的沉默核苷酸取代。苍白球亚种苍白球菌株这种序列改变在非梅毒菌株中引入了PleI限制性位点，从而允许从T.苍白球亚种苍白球菌株
Previous investigations have demonstrated that immunization with Treponema pallidum subsp. pallidum glycerophosphodiester phosphodiesterase significantly protects rabbits from subsequent treponeme challenge. In this report, we show that the glycerophosphodiester phosphodiesterase amino acid sequence is conserved among 12 strains from a total of five pathogenic treponemes. The invariant nature of this immunoprotective antigen makes it an attractive candidate for inclusion in a universal subunit vaccine against T. pallidum infection. In addition, these studies show a silent nucleotide substitution at position 579 of the gpd open reading frame which is consistently observed in the non-T. pallidum subsp. pallidum strains. This sequence alteration introduces a PleI restriction site in the nonsyphilis strains and thus allows genetic differentiation from T. pallidum subsp. pallidum strains.