Neoxanthin and fucoxanthin induce apoptosis in PC-3 human prostate cancer cells
Neoxanthin and fucoxanthin induce apoptosis in PC-3 human prostate cancer cells
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DOI：
10.1016/j.canlet.2004.07.048
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发表时间：
2005-03-18
期刊：
影响因子：
9.7
通讯作者：
Nagao, A
中科院分区：
文献类型：
--
作者：
Kotake-Nara, E;Asai, A;Nagao, A
新黄质和岩藻黄质具有 5,6-单环氧化物和联烯键的特征结构，此前已发现在测试的 15 种膳食类胡萝卜素中，它们能最有效地降低人类前列腺癌细胞的活力。在本研究中，这两种类胡萝卜素诱导 PC-3 细胞凋亡的特点是形态变化、DNA 片段化、亚二倍体细胞百分比增加以及 caspase-3 和 PARP 裂解。 20μM类胡萝卜素处理48h后，凋亡细胞比例达到30％以上。他们减少了 Bax 和 Bcl-2 蛋白的表达，但不减少 Bcl-X-L。岩藻黄质在细胞中的积累水平与新黄质相同。此外，岩藻黄质的脱乙酰化产物岩藻黄质在用岩藻黄质处理的细胞中形成，并在孵育24小时后达到与岩藻黄质相当的水平。单独使用岩藻黄质醇处理也可诱导 PC-3 细胞凋亡。因此，发现新黄质和岩藻黄质治疗可通过激活 PC-3 人前列腺癌细胞中的 caspase-3 来诱导细胞凋亡。 (c) 2004 Elsevier Ireland Ltd. 保留所有权利。
Neoxanthin and fucoxanthin, which have the characteristic structure of 5,6-monoepoxide and an allenic bond, were previously found to reduce the viability of human prostate cancer cells most intensively among 15 dietary carotenoids tested. In the present study, the induction of apoptosis in PC-3 cells by these two carotenoids was characterized by morphological changes, DNA fragmentation, an increased percentage of hypodiploid cells, and cleavages of caspase-3 and PARP. The ratio of apoptotic cells reached more than 30% after treatment for 48 h with 20 mu M carotenoids. They reduced the expression of Bax and Bcl-2 proteins, but not Bcl-X-L. Fucoxanthin accumulated in the cells at the same level as neoxanthin. Moreover, fucoxanthinol, a deacetylated product of fucoxanthin, formed in the cells treated with fucoxanthin and reached a level comparable to that of fucoxanthin after incubation for 24 h. Treatment by fucoxanthinol alone also induced apoptosis in PC-3 cells. Thus, neoxanthin and fucoxanthin treatments were found to induce apoptosis through caspase-3 activation in PC-3 human prostate cancer cells. (c) 2004 Elsevier Ireland Ltd. All rights reserved.