CHARACTERIZATION OF LOW-MOLECULAR-WEIGHT RNAS ASSOCIATED WITH 70S RNA OF ROUS-SARCOMA VIRUS
CHARACTERIZATION OF LOW-MOLECULAR-WEIGHT RNAS ASSOCIATED WITH 70S RNA OF ROUS-SARCOMA VIRUS
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DOI：
10.1128/jvi.12.2.334-342.1973
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发表时间：
1973-01-01
影响因子：
5.4
通讯作者：
GOODMAN, HM
中科院分区：
文献类型：
--
作者：
FARAS, AJ;GARAPIN, AC;GOODMAN, HM
两种低分子量RNA与劳斯肉瘤病毒的70SRNA复合物相关：先前描述的4SRNA和新鉴定的5SRNA。4SRNA占70SRNA复合物的3%至4%，或相当于每70SRNA 12至20个分子。它表现出一些结构特性的转移RNA所揭示的二维电泳的寡核苷酸从T1核糖核酸酶消化的4SRNA物种。5SRNA的长度约为120个核苷酸，占70SRNA复合物的1%或相当于每分子70SRNA 3至4个分子，并且在核苷酸组成和结构上与来自未感染的鸡胚成纤维细胞的5SRNA相同。解链研究表明，5SRNA从70SRNA复合物中释放出来的温度与70SRNA分解成35S亚基所需的温度相同。相比之下，超过80%的4SRNA在转化为亚基之前从70SRNA释放。这些70S相关的RNA的可能的生物学意义进行了讨论。
Two low-molecular-weight RNAs are associated with the 70SRNA complex of Rous sarcoma virus: a previously described 4SRNA and a newly identified 5SRNA. The 4SRNA constitutes 3 to 4% of the 70SRNA complex or the equivalent of 12 to 20 molecules per 70SRNA. It exhibits a number of structural properties characteristic of transfer RNA as revealed by two-dimensional electrophoresis of oligonucleotides obtained from a T1 ribonuclease digest of the 4SRNA species. The 5SRNA is approximately 120 nucleotides in length, constitutes 1% of the 70SRNA complex or the equivalent of 3 to 4 molecules per molecules of 70SRNA, and is identical in nucleotide composition and structure to 5SRNA from uninfected chicken embryo fibroblasts. Melting studies indicate that the 5SRNA is released from the 70SRNA complex at the same temperature required to dissociate 70SRNA into its constituent 35Ssubunits. In contrast, greater than 80% of the 4SRNA is released from 70SRNA prior to its conversion into subunits. The possible biological significance of these 70S-associated RNAs is discussed.