Plant stress hormones suppress the proliferation and induce apoptosis in human cancer cells
Plant stress hormones suppress the proliferation and induce apoptosis in human cancer cells
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DOI：
10.1038/sj.leu.2402419
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发表时间：
2002-04-01
期刊：
影响因子：
11.4
通讯作者：
Flescher, E
中科院分区：
文献类型：
--
作者：
Fingrut, O;Flescher, E
细胞应激源导致多种结果，包括抑制细胞增殖和细胞死亡。水杨酸钠(SA)是一种植物应激激素，可抑制某些哺乳动物癌细胞的增殖或诱导其凋亡。植物应激激素是细胞反应的激活剂，包括细胞死亡，以适应植物中不同的逆境。因此，我们假设植物应激激素具有对癌细胞产生不利影响的能力。我们发现植物应激激素SA抑制淋巴细胞性白血病、前列腺癌、乳腺癌和黑色素瘤人类癌细胞的增殖。茉莉酸(JA)是茉莉酸家族的一种植物应激激素，可诱导淋巴细胞性白血病细胞死亡，并抑制上述其他人类癌细胞的增殖。茉莉酸家族的另一成员，茉莉酸甲酯(MJ)，在每个细胞系中都能诱导死亡。植物应激激素对正常人淋巴细胞没有影响，而对淋巴母细胞白血病细胞有很强的作用。JA和MJ引起了细胞的凋亡性死亡，这是通过核形态特征、流式细胞仪DNA图谱和caspase-3活性升高来确定的。最后，携带EL-4淋巴瘤的小鼠经MJ治疗后，存活时间明显长于未治疗的小鼠(P=0.00953)。这些发现表明，植物应激激素可能是一类新的抗癌药物。
Cellular stressors induce various outcomes including inhibition of cell proliferation and cell death. Sodium salicylate (SA), a plant stress hormone, can suppress the proliferation or cause apoptosis in certain mammalian cancer cells. Plant stress hormones are activators of cellular responses, including cell death, to diverse stress situations in plants. Thus, we hypothesized that plant stress hormones share the ability to adversely affect cancer cells. We found that the plant stress hormone SA suppressed proliferation of lymphoblastic leukemia, prostate, breast and melanoma human cancer cells. Jasmonic acid (JA), a plant stress hormone belonging to the Jasmonate family, induced death in lymphoblastic leukemia cells and caused suppression of cell proliferation in the other human cancer cells mentioned above. Another member of the Jasmonate family, methyl jasmonate (MJ), induced death in each of the cell lines. Plant stress hormones did not affect normal human lymphocytes, in contrast to their strong effect on lymphoblastic leukemia cells. JA and MJ caused apoptotic death, as determined by characteristic nuclear morphology, flow cytometric DNA profile and elevation of caspase-3 activity. Finally, mice bearing EL-4 lymphoma and treated with MJ, survived for significantly (P=0.00953) longer periods of time than untreated mice. These findings suggest that plant stress hormones may potentially be a novel class of anti-cancer drugs.