Characterization of yeast and filamentous fungi from Broggerbreen glaciers, Svalbard
Characterization of yeast and filamentous fungi from Broggerbreen glaciers, Svalbard
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斯瓦尔巴特群岛布罗格布林冰川酵母和丝状真菌的特征
DOI：
10.1017/s0032247416000085
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发表时间：
2016
期刊：
影响因子：
0.8
通讯作者：
Roy U
中科院分区：
文献类型：
--
作者：
Singh P;Tsuji M;Roy U
低温球藻洞穴具有重要的生态和生物技术意义。本文介绍了从斯瓦尔巴特群岛奥特雷冰川和维斯特布鲁格布赖恩冰川的冷孢子洞中分离到的可培养的嗜冷酵母和丝状真菌的研究结果。根据DNA序列分析，鉴定为红酵母属(Rhodotorula sp.)、球孢霉属(Thelebolus sp.)和四链孢霉属(Articospora Etracladia)。其中，四条链霉菌是一个新种，与5.8S rDNA的同源性为88.7-89.4%。丝状真菌节孢霉。酵母菌Cry-FB1和Cry-FB2表达了较高的淀粉酶、纤维素酶、脂肪酶和蛋白水解酶活性。CrY-FB3具有较高的淀粉酶和纤维素酶活性。Thelebolus sp.Cry-YB 240和Cry-YB 241具有较强的蛋白酶和尿素酶活性。研究了温度和盐分对菌体生长的影响。最适生长温度为10℃，最适pH为7.0。冰孔中的丝状真菌和酵母菌可能通过分泌适应低温的酶来驱动有机大分子的降解过程，从而帮助这些冰上环境中的营养循环。此外，这些嗜冷菌由于其产酶能力，可能为北极的生物技术研究提供机会。
Cryoconite holes have ecological and biotechnological importance. This article presents results on culturable cryophilic yeasts and filamentous fungi isolated from cryoconite holes at Austre and Vestre Brøggerbreen glaciers, Svalbard. Based on DNA sequence data, these were identified as Rhodotorula sp., Thelebolus sp., and Articulospora tetracladia. Amongst these, Articulospora tetracladia (88.7–89.4% gene similarity with 5.8S rDNA) is a novel species, yet to be described. Filamentous fungus Articulospora sp. Cry-FB1 and Cry-FB2, expressed high amylase, cellulase, lipase and protease activities while yeast Rhodotorula sp. Cry-FB3 showed high amylase and cellulase activity. Thelebolus sp. Cry-YB 240 and Cry-YB 241 showed protease and urease activities. The effects of temperature, and salt on the growth of the cultures were studied. Optimum temperature of growth was on 10ºC at pH 7.0. Filamentous fungi and yeast in the cryoconite holes possibly drive the process of organic macromolecule degradation through cold-adapted enzyme secretion, thereby assisting in nutrient cycling in these supraglacial environments. Further, these cryophilic fungi, due to their enzyme producing ability, may provide an opportunity for biotechnological research in the Arctic.