MiR-1273 g-3p Promotes Malignant Progression and has Prognostic Implications in Prostate Cancer
MiR-1273 g-3p Promotes Malignant Progression and has Prognostic Implications in Prostate Cancer
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DOI：
10.1007/s12033-021-00384-x
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发表时间：
2021-08-24
影响因子：
2.6
通讯作者：
Sun, Yi
中科院分区：
文献类型：
--
作者：
Chang, Yaxue;Deng, Qian;Sun, Yi
前列腺癌（PCa）是人类最常见的癌症。本研究旨在探讨miR-1273 g-3p在前列腺癌中的表达及其预测意义。此外，还研究了对细胞生物学活性的影响。通过实时定量PCR验证miR-1273 g-3p在PCa组织和细胞系中的相对表达。采用Kaplan-Meier曲线和考克斯回归分析判断预后。使用CCK-8和Transwell试验验证了miR-1273 g-3 p对细胞增殖、迁移和侵袭的影响。我们的研究结果表明，miR-1273 g-3p在PCa组织和细胞系中的表达增加。miR-1273 g-3p表达水平与Gleason评分、TNM分期、临床分期和淋巴结转移相关。miR-1273 g-3p的过表达表明有希望的总体存活率。考克斯回归分析结果提示miR-1273 g-3p可能是PCa患者的独立标志物。沉默的miR-1273 g-3p抑制PCa细胞增殖、迁移和侵袭。总之，miR-1273 g-3p在PCa中增加，并被鉴定为PCa患者的治疗靶点和预后因素。miR-1273 g-3p的过表达可能是通过加速细胞增殖、迁移和侵袭的癌基因。
Prostate cancer (PCa) is the most popular cancer of mankind. Our study aimed to provide the expression and the predictive significance of miR-1273 g-3p in PCa. Moreover, the effects on cell biological activities were also investigated. The relative expression of miR-1273 g-3p in PCa tissues and cell lines was validated by quantitative real-time PCR. Kaplan-Meier curve and Cox regression analyses were performed to indicate the prognostic value. The implications of miR-1273 g-3p on cell proliferation, migration, and invasion were validated using the CCK-8 and Transwell assay. Our results provided that the expression of miR-1273 g-3p was increased in PCa tissues and cell lines. The levels of miR-1273 g-3p were associated with Gleason score, TNM stage, clinical stage, and lymph node metastasis. Overexpression of miR-1273 g-3p indicated a promising overall survival rate. Cox regression results indicated miR-1273 g-3p might be an independent marker for PCa patients. Silenced miR-1273 g-3p inhibited PCa cell proliferation, migration, and invasion. In total, miR-1273 g-3p was increased in PCa and identified as a therapeutic target and a prognostic factor for PCa patients. Overexpression of miR-1273 g-3p might be an oncogene via accelerating cell proliferation, migration, and invasion.