JUXTACELLULAR LABELING OF TONICALLY ACTIVE NEURONS AND PHASICALLY ACTIVE NEURONS IN THE RAT STRIATUM
JUXTACELLULAR LABELING OF TONICALLY ACTIVE NEURONS AND PHASICALLY ACTIVE NEURONS IN THE RAT STRIATUM
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DOI：
10.1016/j.neuroscience.2010.03.062
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发表时间：
2010-06-30
期刊：
影响因子：
3.3
通讯作者：
Kimura, M.
中科院分区：
文献类型：
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作者：
Inokawa, H.;Yamada, H.;Kimura, M.
根据在体细胞内记录结合麻醉大鼠的形态学检查，以及组织化学、电生理和体外切片制备的标记研究，普遍认为纹状体中的紧张性活动神经元（TANs）和相性活动神经元（PANs）分别是胆碱能中间神经元和GABA能投射神经元。警觉行为动物的TAN对行为上重要的事件表现出延长的暂停反应。另一方面，当受试者安静且不执行任何动作时，PAN大多是不活动的，但响应于外部刺激和/或自主动作而表现出爆发性放电。在纹状体中还鉴定了几种其他类型的中间神经元，例如含小清蛋白的GABA能中间神经元（快速尖峰细胞）、含生长抑素的中间神经元和含钙视黄蛋白的中间神经元。为了更直接地识别TAN和PAN的神经化学和形态学特征，我们在麻醉大鼠中进行了神经细胞标记，结合电生理学、免疫组织化学和形态学。在记录的那些（n=10）中，所有的细胞标记的TAN（n=3）都是ChAT阳性的，并且具有带刺状树突的大细胞胞体。因此，虽然我们的观察是基于有限数量的神经元，我们的研究结果提供了迄今为止最令人信服的证据，证明纹状体中的TAN是胆碱能神经元。我们还发现，大多数PAN是GABA免疫反应性的（48个测试中的46个），大约三分之二有棘状树突（48个测试中的30个），这表明大多数是中等大小的，多刺的，GABA能投射神经元，与一般的信念一致。相反，其余三分之一的PAN有刺状树突（n=18），表明它们是中间神经元。因此，本研究表明，TAN是胆碱能神经元，大多数PAN是中等大小的，多刺的，GABA能投射神经元，而少数是GABA能中间神经元。(C)2010年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Tonically active neurons (TANs) and phasically active neurons (PANs) are widely believed to be the cholinergic interneurons and GABAergic projection neurons, respectively, in the striatum based on in vivo intracellular recordings coupled with morphological examinations of anesthetized rats, and on histochemical, electrophysiological, and labeling studies of in vitro slice preparations. TANs of alert behaving animals exhibit prolonged pause responses to behaviorally significant events. PANs, on the other hand, are mostly inactive when subjects are quiet and not performing any actions, but exhibit burst discharges in response to external stimuli and/or voluntary actions. Several other types of interneurons have also been identified in the striatum, such as parvalbumin-containing GABAergic interneurons (fast-spiking cells), somatostatin-containing interneurons, and calretinin-containing interneurons. To identify the neurochemical and morphological characteristics of TANs and PANs in a more direct manner, we conducted juxtacellular labeling, combining electrophysiology with immunohistochemistry and morphology in anesthetized rats. All of the juxtacellularly labeled TANs (n=3) among those recorded (n=10) were ChAT-positive and had large cell somata with aspiny dendrites. Thus, although our observations are based on a limited number of neurons, our findings provide the most convincing evidence to date that TANs in the striatum are cholinergic neurons. We also found that the majority of PANs are GABA-immunoreactive (46 of 48 tested) and approximately two-thirds had spiny dendrites (30 of 48 tested), indicating that the majority are medium-sized, spiny, GABAergic projection neurons, consistent with general beliefs. Conversely, the remaining one-third of PANs had aspiny dendrites (n=18), indicating that they were interneurons. Therefore, the present study reveals that TANs are cholinergic neurons and that the majority of PANs are medium-sized, spiny, GABAergic projection neurons, while a smaller number are GABAergic interneurons. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.