IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF A CHITIN BINDING-LIKE PROTEIN FROM FENNEROPENAEUS CHINENSIS (OSBECK, 1765)
IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF A CHITIN BINDING-LIKE PROTEIN FROM FENNEROPENAEUS CHINENSIS (OSBECK, 1765)
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DOI：
10.1163/156854012x650250
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发表时间：
2012-08-01
期刊：
影响因子：
0.6
通讯作者：
Wang, Q. Y.
中科院分区：
文献类型：
--
作者：
Lai, X. F.;Gao, H.;Wang, Q. Y.
本研究从中国明对虾Fenneropenaeus chinensis（Osbeck，1765）中克隆了一个几丁质结合样蛋白，命名为FcCBP。结果表明，该基因全长2098 bp，含4个内含子，开放阅读框编码190个氨基酸。成熟蛋白的计算分子量为20.2 kDa，理论pI为5.93。FcCBP共有一个保守的ChtBD 2结构域（氨基酸83-154）和六个保守的半胱氨酸（33、75、85、124、127和138）。系统发育分析表明，FcCBP与其他无脊椎动物的CBP同源性较低。qRT-PCR分析表明，FcCBP在肠道、肝胰腺、肌肉和鳃组织中均有表达，且在WSSV（白色斑点综合征病毒）攻击后肝胰腺和鳃组织中表达量显著增加，随后又呈下降趋势。这些结果表明，FcCBP可能参与了华绒螯蟹对WSSV的免疫防御反应。
In this study, a chitin-binding-like protein was cloned and characterized from Fenneropenaeus chinensis (Osbeck, 1765) and named as FcCBP. The results indicated that the full length DNA of 2098 bp with four introns, had an open reading frame encoding a polypeptide of 190 amino acids. The calculated molecular mass of the mature protein was 20.2 kDa and the theoretical pI was 5.93. The FcCBP shared a conserved ChtBD2 domain (amino acids 83-154) with six conserved cysteines (33, 75, 85, 124, 127 and 138). A phylogenetic analysis of CBP sequences showed that FcCBP had low homology with the CBPs from other invertebrate groups. qRT-PCR analysis indicated that FcCBP was expressed in intestine, hepatopancreas, muscle, and gill tissues, and its profile was increased significantly post WSSV (White Spot Syndrome Virus) challenge in the hepatopancreas and gills, followed again by a decreasing trend. These results indicate that FcCBP might be involved in the immune defense response to WSSV in E chinensis.